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RHOA Monoclonal Antibody (1C11)

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RHOA Antibody (H00000387-M07) in WB

Western Blot analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M07), clone 1C11.

Lane 1: RHOA transfected lysate(21.8 KDa).
Lane 2: Non-transfected lysate.

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产品信息

H00000387-M07

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

酶联免疫吸附实验 (ELISA)

1-5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, lambda

分类

Monoclonal

类型

Antibody

克隆号

1C11

抗原

RHOA (AAH01360, 1 a.a. approximately 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

See Label

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Sequence of this protein is as follows: MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRNDEHTRRE LAKMKQEPVK PEEGRDMANR IGAFGYMECS AKTKDGVREV FEMATRAALQ ARRGKKKSGC LVL

靶标信息

RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Aplysia ras-related homolog 12; h12; MGC72339; oncogene RHO H12; Rho; Rho cDNA clone 12; small GTP binding protein RhoA; Transforming protein RhoA

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基因别名: ARH12; ARHA; RHO12; RHOA; RHOH12

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UniProt ID: (Human) P61586

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Entrez Gene ID: (Human) 387

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功能

GTPase activity protein binding GTP binding myosin binding small GTPase

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