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RNF20 Antibody (CF807609) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF20 (RC218422, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF20. Positive lysates LY412703 (100 µg) and LC412703 (20 µg) can be purchased separately from OriGene.
产品信息
CF807609
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
OTI13A10
抗原
Human recombinant protein fragment corresponding to amino acids 493-754 of human RNF20 produced in E.coli.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 8% trehalose
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BRE1 E3 ubiquitin ligase homolog; BRE1-A; E3 ubiquitin-protein ligase BRE1A; FLJ11189; FLJ20382; HNF3B; homolog of S. cerevisiae BRE1; KIAA2779; MGC129667; MGC129668; RING finger protein 20; RING-type E3 ubiquitin transferase BRE1A; TCF3B
基因别名: BRE1; BRE1A; hBRE1; RNF20
UniProt ID: (Human) Q5VTR2
Entrez Gene ID: (Human) 56254
protein polyubiquitination
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
regulation of mitotic cell cycle
histone monoubiquitination
negative regulation of cell migration
positive regulation of histone methylation
histone H2B ubiquitination
positive regulation of transcription, DNA-templated
negative regulation of mRNA polyadenylation
positive regulation of histone H2B ubiquitination
