Invitrogen

RPA70 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3个已发表图片

2 篇参考文献

查看其他19个RPA70抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

RPA70 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

RPA70 Antibody (PA5-21976) in ICC/IF

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit antibody (Product # PA5-21976) at 1:200 dilution.

RPA70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

RPA70 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RPA70 siRNA and decrease in signal was observed in Western Blot using RPA70 Polyclonal Antibody (Product # PA5-21976). {KD}

产品信息

PA5-21976

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 369 and 616 of RPA70 (Uniprot ID#P27694)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.4 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_11154806

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse liver.

Predicted reactivity: Mouse (87%), Rat (85%), Sheep (93%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Maternal Smc3 protects the integrity of the zygotic genome through DNA replication and mitosis.

Development (Cambridge, England)

Yueh WT,Singh VP,Gerton JL

PA5-21976 was used in Immunocytochemistry-immunofluorescence to suggest that, despite previous reports of aneuploidy in early embryos, chromosome missegregation in zygotes halts embryogenesis at the two-cell stage.

Wed Dec 15 00:00:00 EST 2021

Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells.

Nature

Flach J,Bakker ST,Mohrin M,Conroy PC,Pietras EM,Reynaud D,Alvarez S,Diolaiti ME,Ugarte F,Forsberg EC,Le Beau MM,Stohr BA,Méndez J,Morrison CG,Passegué E

PA5-21976 was used in immunocytochemistry to study why HSC function declines with age

Thu Aug 14 00:00:00 EDT 2014

Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells.

Nature

Flach J,Bakker ST,Mohrin M,Conroy PC,Pietras EM,Reynaud D,Alvarez S,Diolaiti ME,Ugarte F,Forsberg EC,Le Beau MM,Stohr BA,Méndez J,Morrison CG,Passegué E

PA5-21976 was used in immunocytochemistry to study why HSC function declines with age

Thu Aug 14 00:00:00 EDT 2014

生物信息学

蛋白别名: MSTP075; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; replication protein A1, 70kDa; RF-A protein 1; RP-A p70; Single-stranded DNA-binding protein

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基因别名: 5031405K23Rik; 70kDa; AA589576; AW557552; HSSB; MST075; REPA1; RF-A; RP-A; Rpa; RPA1; RPA70

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UniProt ID: (Human) P27694, (Mouse) Q8VEE4

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Entrez Gene ID: (Human) 6117, (Mouse) 68275

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功能

damaged DNA binding single-stranded DNA binding protein binding metal ion binding nucleic acid binding DNA binding chromatin binding DNA metabolism protein

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