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Invitrogen

RPSA Monoclonal Antibody (MLuC5)

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10 篇参考文献

查看其他19个RPSA抗体

说明书

实验方案

技术支持

货号 MA5-12840

CNY 5,007.00

500 µL

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说明书

实验方案

技术支持

抗体检测数据 (4)
已发表图片 (1)

Application

RPSA Antibody in Immunocytochemistry (ICC/IF)

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RPSA Antibody (MA5-12840) in ICC/IF

Immunofluorescent analysis of Laminin Receptor (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Laminin Receptor monoclonal antibody (Product # MA5-12840) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated... View More

RPSA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-12840

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10-1:200

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

4-8 µg/mL

免疫细胞化学 (ICC/IF)

1:10-1:200

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流式细胞分析 (Flow)

Assay-dependent

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中和实验 (Neu)

查看3篇已发表文章 3篇已发表文章

阻断反应 (BLOCK)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

MLuC5

抗原

Small cell lung carcinoma N592 live cells

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偶联物

Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10983211

产品详细信息

MA5-12840 targets Laminin Receptor in FACS, WB, ICC/IF, and IHC (P) applications and shows reactivity with Human samples.

The MA5-12840 immunogen is small cell lung carcinoma N592 live cells.

靶标信息

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The effects of cytotoxic necrotizing factor 1 expression in the uptake of Escherichia coli K1 by macrophages and the onset of meningitis in newborn mice.

Virulence

Chang AC,Krishnan S,Prasadarao NV

MA5-12840 was used in western blot to study the uptake of Escherichia coli K1 by macrophages and the onset of meningitis in newborn mice and the effects of cytotoxic necrotizing factor 1 expression

Sun Oct 02 00:00:00 UTC 2016

Inhibition of EMMPRIN and MMP-9 Expression by Epigallocatechin-3-Gallate through 67-kDa Laminin Receptor in PMA-Induced Macrophages.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wang QM,Wang H,Li YF,Xie ZY,Ma Y,Yan JJ,Gao YF,Wang ZM,Wang LS

MA512840 was used in blocking or activating experiment and western blot to investigate the effect of epigallocatechin-3-gallate treatment on EMMPRIN and MMP-9 expression in PMA-induced macrophages

Mon Feb 06 00:00:00 UTC 2017

Green tea polyphenol epigallocatechin-3-gallate inhibits TNF-α-induced production of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wang ZM,Gao W,Wang H,Zhao D,Nie ZL,Shi JQ,Zhao S,Lu X,Wang LS,Yang ZJ

MA5-12840 was used in blocking or activating experiment to study the roles of NFkB and the 67-kD laminin receptor in the mechanism by which a green tea polyphenol inhibits TNF-alpha-induced MCP-1 production by HUVECs

Tue Apr 14 00:00:00 UTC 2015

Enteric-delivered rapamycin enhances resistance of aged mice to pneumococcal pneumonia through reduced cellular senescence.

Experimental gerontology

Hinojosa CA,Mgbemena V,Van Roekel S,Austad SN,Miller RA,Bose S,Orihuela CJ

MA5-12840 was used in western blot to study the role of lowered lung cellular senescence in the mechanism by which enteric rapamycin reduces the susceptibility of aged mice to Streptococcus pneumoniae infection

Sat Dec 01 00:00:00 UTC 2012

Green tea polyphenol EGCG sensing motif on the 67-kDa laminin receptor.

PloS one

Fujimura Y,Sumida M,Sugihara K,Tsukamoto S,Yamada K,Tachibana H

MA5-12840 was used in western blot to study the functional domain of 67kDa laminin responsible for the anti-cancer activity of the green tea polyphenol (-)-epigallocatechin-3-O-gallate

Mon Oct 29 00:00:00 UTC 2012

Green tea polyphenol epigallocatechin-3-gallate inhibits TLR2 signaling induced by peptidoglycan through the polyphenol sensing molecule 67-kDa laminin receptor.

FEBS letters

Byun EH,Omura T,Yamada K,Tachibana H

MA5-12840 was used in blocking or activating experiment to study the molecular mechanism for the inhibition of peptidoglycan-dependent TLR2 signalling by a Green tea polyphenol.

Wed Mar 09 00:00:00 UTC 2011

High expression of 67-kDa laminin receptor relates to the proliferation of leukemia cells and increases expression of GM-CSF receptor.

Experimental hematology

Ando K,Miyazaki Y,Sawayama Y,Tominaga S,Matsuo E,Yamasaki R,Inoue Y,Iwanaga M,Imanishi D,Tsushima H,Fukushima T,Imaizumi Y,Taguchi J,Yoshida S,Hata T,Tomonaga M

MA5-12840 was used in flow cytometry to study the role of high level expression of the 67-kDa laminin receptor in GM-CSF receptor expression and proliferation of leukemia cells

Tue Feb 01 00:00:00 UTC 2011

Relationship of up-regulation of 67-kd laminin receptor to grade of cervical intraepithelial neoplasia and to high-risk HPV types and prognosis in cervical cancer.

Acta cytologica

Branca M,Giorgi C,Ciotti M,Santini D,Di Bonito L,Costa S,Benedetto A,Bonifacio D,Di Bonito P,Paba P,Accardi L,Mariani L,Syrjänen S,Favalli C,Syrjänen K

MA5-12840 was used in immunohistochemistry to investigate the prognostic value of the 67-kd laminin receptor in cervical cancer and its relationship with high-risk HPV types

Thu Mar 23 00:00:00 UTC 2006

Increase in laminin expression in allergic airway remodelling and decrease by dexamethasone.

The European respiratory journal

Christie PE,Jonas M,Tsai CH,Chi EY,Henderson WR

MA5-12840 was used in immunocytochemistry to study the role of laminin on allergic airway remodelling in chronic allergic lung inflammation.

Thu Jul 01 00:00:00 UTC 2004

Human T cells express a functional ionotropic glutamate receptor GluR3, and glutamate by itself triggers integrin-mediated adhesion to laminin and fibronectin and chemotactic migration.

Journal of immunology (Baltimore, Md. : 1950)

Ganor Y,Besser M,Ben-Zakay N,Unger T,Levite M

MA5-12840 was used in blocking or activating experiment to study the role of GluR3 in the glutamate triggered adhesion and chemotactic migration of human T cells

Tue Apr 15 00:00:00 UTC 2003

生物信息学

蛋白别名: 37 kDa laminin receptor; 37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRP; 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; Colon carcinoma laminin-binding protein; Laminin receptor 1; laminin receptor 1 (67kD, ribosomal protein SA); Laminin-binding protein precursor p40; LamR; LBP/p40; LRP/LR; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; rssa; Small ribosomal subunit protein uS2

基因别名: 37LRP; 67LR; ICAS; LAMBR; lamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; NEM/1CHD4; p40; RPSA; SA

UniProt ID: (Human) P08865

Entrez Gene ID: (Human) 3921

功能

virus receptor activity structural constituent of ribosome laminin receptor activity protein binding ribosome binding laminin binding poly(A) RNA binding ribosomal protein

参与通路

ribosomal small subunit assembly nuclear-transcribed mRNA catabolic process, nonsense-mediated decay endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA processing rRNA export from nucleus translation translational initiation SRP-dependent cotranslational protein targeting to membrane cell adhesion viral transcription viral entry into host cell

RPSA Monoclonal Antibody (MLuC5)

RPSA Antibody (MA5-12840) in ICC/IF

产品信息

MA5-12840

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

中和实验 (Neu)

阻断反应 (BLOCK)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

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RPSA Monoclonal Antibody (MLuC5)

RPSA Antibody (MA5-12840) in ICC/IF

产品信息

MA5-12840

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

中和实验 (Neu)

阻断反应 (BLOCK)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID