Invitrogen

RTN4RL2 Polyclonal Antibody

RTN4RL2 Antibody in Immunocytochemistry (ICC/IF)

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RTN4RL2 Antibody (PA5-98577) in ICC/IF

Immunofluorescent analysis of RTN4RL2 in MCF-7 cells using a RTN4RL2 polyclonal antibody (Product # PA5-98577) at a dilution of 1:50. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

RTN4RL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RTN4RL2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

PA5-98577

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

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免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

Assay-Dependent

免疫沉淀 (IP)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide sequence from Human Reticulon-4 receptor-like 2 protein (355-372AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.12 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_2813190

靶标信息

Reticulon 4 receptor-like 2 (RTN4RL2), also known as NgR2and NgRH1, is a 60 kDa glycosylphosphatidylinositol-anchored protein that is expressed primarily in the brain. It contains eight leucine-rich repeats that are flanked by cysteine-rich sequences at both the N- and C-termini. The membrane anchored RTN4RL2 can be solubilized through the action of phospholipase or an unidentified MTMMP to generate a 46 kDa soluble receptor. It has been suggested that RTN4RL2 may play a role in regulating axonal regeneration and plasticity in the adult central nervous system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The NOGO receptor NgR2, a novel αVβ3 integrin effector, induces neuroendocrine differentiation in prostate cancer.

Scientific reports

Quaglia F,Krishn SR,Sossey-Alaoui K,Rana PS,Pluskota E,Park PH,Shields CD,Lin S,McCue P,Kossenkov AV,Wang Y,Goodrich DW,Ku SY,Beltran H,Kelly WK,Corey E,Klose M,Bandtlow C,Liu Q,Altieri DC,Plow EF,Languino LR

PA5-98577 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot, Immunoprecipitation to show a novel pathway activated by αVβ3 that promotes NE differentiation (NED).