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RUNX1/RUNX1T1 Antibody (PA5-40076) in ChIP-seq
ChIP assays were performed on Kasumi-1 cells using a AML1-ETO polyclonal antibody (Product # PA5-40076) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 µl of antibody were used per ChIP experiment. QPCR was performed on primers specific for the FUT7, NFE2 and OGG1 genes. The IP'd DNA of 6 ChIPs was pooled and analyzed with a Genome Analyzer. The 32 bp tags were aligned t... View More
产品信息
PA5-40076
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide corresponding to human AML1-ETO fusion protein.
偶联物
Unconjugated
形式
Liquid
浓度
Conc. Not Determined
保存液
whole serum
内含物
0.05% sodium azide
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2608182
靶标信息
AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in all tissues examined except brain and heart, and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: acute myelogenous leukemia 1 translocation 1, cyclin-D related; Acute myeloid leukemia 1 protein; aml1 oncogene; AML1-EVI-1 fusion protein; CBF-alpha-2; CBFA2T1 isoform r1t1-11a61; CBFA2T1 isoform r1t1-11a62; CBFA2T1 isoform r1t1-11a63; CBFA2T1 isoform r1t1-11a64; CBFA2T1 isoform r1t1-11a65; CBFA2T1 isoform r1t1-7a47; CBFA2T1 isoform r1t1-7a48; CBFA2T1 isoform r1t1-7a49; CBFA2T1 isoform r1t1-7a50; CBFA2T1 isoform r1t1-7a51; CBFA2T1 isoform r1t1-7a52; CBFA2T1 isoform r1t1-7d53; CBFA2T1 isoform r1t1-7d54; CBFA2T1 isoform r1t1-7d55; CBFA2T1 isoform r1t1-7d56; CBFA2T1 isoform r1t1-8a57; CBFA2T1 isoform r1t1-8a58; CBFA2T1 isoform r1t1-8a59; CBFA2T1 isoform r1t1-8a60; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related; Cyclin-D-related protein; Eight twenty one protein; myeloid translocation gene on 8q22; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; Protein CBFA2T1; Protein ETO; Protein MTG8; runt related transcription factor 1; translocated to, 1 (cyclin D related); Runt-related transcription factor 1; runt-related transcription factor 1; translocated to, 1 (cyclin D-related); RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor; Zinc finger MYND domain-containing protein 2
基因别名: AML1; AML1-EVI-1; AML1-MTG8; AML1T1; AMLCR1; CBF2alpha; CBFA2; CBFA2T1; CDR; ETO; EVI-1; MTG8; PEBP2aB; PEBP2alpha; RUNX1; RUNX1T1; ZMYND2
UniProt ID: (Human) Q01196, (Human) Q06455
Entrez Gene ID: (Human) 861, (Human) 862
regulatory region DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
ATP binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
metal ion binding
transcription cofactor
DNA-binding transcription factor
immunoglobulin fold transcription factor
Runt transcription factor
gene-specific transcriptional regulator
ossification
chondrocyte differentiation
transcription from RNA polymerase II promoter
hemopoiesis
positive regulation of granulocyte differentiation
positive regulation of interleukin-2 production
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
peripheral nervous system neuron development
hematopoietic stem cell proliferation
generation of precursor metabolites and energy
transcription, DNA-templated
negative regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
