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Invitrogen
Rabbit anti-Goat IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor™ 680
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Goat IgG (Heavy Chain) Secondary Antibody (A27020) in WB
Expression of endogenous SM22alpha was detected using the anti-SM22alpha Goat primary antibody and Rabbit anti-Goat IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 680 Conjugate (Product # A27020) (1 µg/mL, 1:1000 dilution). Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa human cervical carcinoma cells (lane 1-3) using the Xcell Surelock Electrophoresis syst... View More
产品信息
A27020
应用
建议稀释比
免疫印迹 (WB)
0.05-2 µg/mL
产品规格
种属反应
Goat
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Recombinant full-length protein
偶联物
激发/发射光谱
681/704 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Gravity column chromatography
保存液
PBS
内含物
5mM sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_2536084
靶标
IgG (Heavy chain)
抗体形式
Whole Antibody
产品详细信息
The sensitivity and specificity of each lot is confirmed using ELISA.
Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
