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Mouse IgG Fc Secondary Antibody (SA5-10211) in WB
Fluorescent western blot was performed using Rabbit anti-Mouse IgG Fc Recombinant Secondary Antibody (Product # SA5-10211). Whole cell extracts of A-431 (Lane 1, 2, 3, 4), HaCaT (Lane 5, 6, 7, 8), SH-SY5Y (Lane 9), HeLa (Lane 10), MCF7 (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto a nitrocellulose membrane (Product # IB... View More
产品信息
SA5-10211
应用
建议稀释比
免疫印迹 (WB)
0.1-0.5 µg/mL
酶联免疫吸附实验 (ELISA)
0.005-0.2 µg/mL
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Secondary Antibody
克隆号
RM104
抗原
Peptide corresponding to Mouse IgG.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4, with 1% BSA, 50% glycerol
内含物
0.09% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2665334
靶标
IgG Fc
抗体形式
Whole Antibody
产品详细信息
This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.
The Fc region of SA-10211 has been engineered to eliminate Fc receptor binding.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
