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Invitrogen
Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight™ 550
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Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (SA5-10159) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate was performed using MCF-7 cells stained with Cytokeratin 19 (RCK108) Mouse Monoclonal Primary Antibody (Product # MA5-12613). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with Mous... View More
产品信息
SA5-10159
应用
建议稀释比
免疫印迹 (WB)
1:5,000-1:20,000
免疫组化（石蜡） (IHC (P))
1:50-1:2,000
免疫细胞化学 (ICC/IF)
1:50-1:500
流式细胞分析 (Flow)
1:50-1:200
免疫沉淀 (IP)
Assay-dependent
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Mouse IgG-heavy and light chain
偶联物
激发/发射光谱
556/570 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 6.8 to 7.4, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2556739
靶标
IgG
交叉吸附
Against human and rat
抗体形式
Whole Antibody
产品详细信息
This antibody is cross-adsorbed and exhibits minimum reactivity to human and rat.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
