Invitrogen

Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP

Mouse IgG (H+L) Secondary Antibody in Western Blot (WB)

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Mouse IgG (H+L) Secondary Antibody (61-6520) in WB

Chemiluminescent western blot was performed using Rabbit anti-Mouse IgG (H+L) Secondary Antibody, HRP (Product # 61-6520). Whole cell extracts of U-87 MG (Lane 1, 2, 3, 4), SH-SY5Y (Lane 5, 6, 7, 8), HeLa (Lane 9), Hep G2 (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More

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产品信息

61-6520

应用

建议稀释比

免疫印迹 (WB)

1:2,000-1:10,000

酶联免疫吸附实验 (ELISA)

1:2,000-1:4,000

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Secondary Antibody

抗原

Gamma Immunoglobin

偶联物

HRP HRP HRP

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 4mg/mL BSA, 40% glycerol

内含物

0.19% Kathon™ CG

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533933

靶标

IgG

抗体形式

Whole Antibody

靶标信息

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

仅用于科研。不用于诊断过程。未经明确授权不得转售。