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Invitrogen
Rat anti-Mouse IgG2a (Heavy chain) Secondary Antibody, HRP
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Mouse IgG2a (Heavy chain) Secondary Antibody (MA5-16790) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat. The blots were probed with Anti LKB1 Mouse Monoclonal Antibody (Product # AHO1392, 2 µg/mL) and detected by chemiluminescence using Rat anti-Mouse IgG2a (Heavy chain) Secondary Antibody, HRP (Product # MA5-16790) at dilutions 1:1,000 (Fig. 1) and 1:5,000 (Fig. 2). A 55 kDa band corresponding to LKB1 was observed. Known quan... View More
产品信息
MA5-16790
应用
建议稀释比
免疫印迹 (WB)
1:1,000-1:5,000
酶联免疫吸附实验 (ELISA)
0.5 µg/mL
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG1
分类
Monoclonal
类型
Secondary Antibody
克隆号
LO-MG2a-7
抗原
Purified IgG from BALB/c mice
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2538273
靶标
IgG2a (Heavy chain)
抗体形式
Whole Antibody
产品详细信息
Rat anti Mouse IgG2a:Biotin, clone LO-MG2a-7 recognizes the gamma 2a heavy chain of mouse immunoglobulin and does not cross-react with other murine immunoglobulin classes or subclasses.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
