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Rb Antibody (MA5-35383) in ICC/IF
Confocal imaging (Immunocytochemistry) of Rb in U-2 OS cells. Samples were incubated with Rb Monoclonal antibody (Product # MA5-35383) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
产品信息
MA5-35383
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ARC0810
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 729-928 of human RB (P06400)
偶联物
形式
Liquid
浓度
1.5 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2849284
产品详细信息
Immunogen sequence: KDLPHAVQET FKRVLIKEEE YDSIIVFYNS VFMQRLKTNI LQYASTRPPT LSPIPHIPRS PYKFPSSPLR IPGGNIYISP LKSPYKISEG LPTPTKMTPR SRILVSIGES FGTSEKFQKI NQMVCNSDRV LKRSAEGSNP PKPLKKLRFD IEGSDEADGS KHLPGESKFQ QKLAEMTSTR TRMQKQKMND SMDTSNKEEK
靶标信息
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; P105RB; p110-RB1; Phospho-Retinoblastoma; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; retinoblastoma gene; retinoblastoma suspectibility protein; Retinoblastoma-associated protein
基因别名: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; RB1
UniProt ID: (Human) P06400
Entrez Gene ID: (Human) 5925
core promoter binding
RNA polymerase II activating transcription factor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription factor binding
kinase binding
ubiquitin protein ligase binding
identical protein binding
androgen receptor binding
phosphoprotein binding
chromatin/chromatin-binding, or -regulatory protein
cell cycle checkpoint
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
chromatin remodeling
transcription, DNA-templated
negative regulation of protein kinase activity
cell cycle arrest
negative regulation of transcription from RNA polymerase II promoter during mitosis
mitotic cell cycle checkpoint
Ras protein signal transduction
regulation of mitotic cell cycle
negative regulation of gene expression
viral process
covalent chromatin modification
androgen receptor signaling pathway
sister chromatid biorientation
neuron projection development
maintenance of mitotic sister chromatid cohesion
glial cell apoptotic process
skeletal muscle cell differentiation
neuron maturation
enucleate erythrocyte differentiation
negative regulation of sequence-specific DNA binding transcription factor activity
regulation of lipid kinase activity
myoblast differentiation
positive regulation of macrophage differentiation
positive regulation of mitotic metaphase/anaphase transition
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
digestive tract development
cell morphogenesis involved in neuron differentiation
negative regulation of epithelial cell proliferation
striated muscle cell differentiation
cell division
neuron apoptotic process
protein localization to chromosome, centromeric region
cellular response to xenobiotic stimulus
regulation of cohesin loading
negative regulation of transcription involved in G1/S transition of mitotic cell cycle
regulation of centromere complex assembly
hepatocyte apoptotic process
negative regulation of G1/S transition of mitotic cell cycle
positive regulation of transcription regulatory region DNA binding
