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Invitrogen
Red Firefly Luciferase Polyclonal Antibody
高级验证
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
图: 1 / 8
Red Firefly Luciferase Antibody (PA1-179) in ICC/IF
Immunofluorescent analysis of Red firefly luciferase (from the Japanese firefly Luciola cruciata) in pCMV-Red Firefly (RFF) Luc transfected (green, left panel) and untransfected (UT) 293 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cel... View More
产品信息
PA1-179
应用
建议稀释比
已发表文章
产品规格
种属反应
Luciferase
已发表种属
Luciferase
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified full length recombinant Red Firefly luciferase expressed in E. coli.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA, 30% glycerol
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539910
产品详细信息
Western blot analysis of PA1-179 detects a prominent ~62 kDa protein in Red Firefly luciferase expressing cells. No signal was detected in Green Renilla transfected or untransfected control HEK293 cells. This antibody has also been successfully tested in IP, Immunofluorescence and neutralization assays.
Red Firefly luciferase activity can be measured using the Thermo Fisher Firefly Luciferase Flash
or Glow Assay Kits or Luciferase Dual Assay Kits.
靶标信息
Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; luciferin 4-monooxygenase; Photinus pyralis; photinus pyralis luciferase
