Osenses

RhoA Polyclonal Antibody

产品信息

OSR00298G-500UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

10-50 µg/mL

免疫组化 (IHC)

10-50 µg/mL

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide from aa region 100-150 of human RhoA conjugated to blue carrier protein was used as the antigen

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

Conc. Not Determined

纯化类型

Ammonium sulfate precipitation

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in many species including rat, mouse, chicken, dog, bovine, frog, zebrafish and orangutan.

Specificity of this antibody: RhOA.

靶标信息

RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells.

Experimental cell research

El-Sibai M,Pertz O,Pang H,Yip SC,Lorenz M,Symons M,Condeelis JS,Hahn KM,Backer JM

Tue Apr 15 00:00:00 EDT 2008

生物信息学

蛋白别名: Aplysia ras-related homolog 12; aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; h12; MGC72339; oncogene RHO H12; plysia ras-related homolog A2; Ras family member A; ras homolog A1; ras homolog A2; ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; Rho; Rho cDNA clone 12; Rho family GTPase; small GTP binding protein RhoA; Transforming protein RhoA

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基因别名: ARH12; ARHA; Arha1; Arha2; RHO12; RHOA; RHOH12

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UniProt ID: (Human) P61586, (Rat) P61589, (Mouse) Q9QUI0

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Entrez Gene ID: (Human) 387, (Rat) 117273, (Mouse) 11848

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功能

GTPase activity protein binding GTP binding myosin binding GDP binding protein domain specific binding Rho GDP-dissociation inhibitor binding nucleotide binding small GTPase

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