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Application
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RhoB Antibody (702242) in ICC/IF
For immunofluorescence analysis, A431 cells were fixed and permeabilized for detection of endogenous RhoB using Anti- RhoB Recombinant Rabbit Monoclonal Antibody (Product # 702242, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of RhoB protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938)... View More
产品信息
702242
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
19H1L5
抗原
Peptides corresponding to Human RHOB (aa 128-145, 50-65)
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633072
产品详细信息
This antibody is predicted to react with Monkey, Mouse, Goat and Cat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Rho-related GTP-binding protein (RhoB) mediates apoptosis in neoplastically transformed cells after DNA damage. While not essential for development, it does affect cell adhesion and growth factor signaling in transformed cells. RhoB targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a tumor supressor - deletion of RhoB causes tumor formation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Aplysia RAS-related homolog 6; h6; oncogene RHO H6; ras homolog gene family, member B; Rho; Rho cDNA clone 6; Rho-related GTP-binding protein RhoB
基因别名: ARH6; ARHB; MST081; MSTP081; RHOB; RHOH6
UniProt ID: (Human) P62745
Entrez Gene ID: (Human) 388
cytokinesis
angiogenesis
intracellular protein transport
apoptotic process
cell adhesion
Rho protein signal transduction
endosome to lysosome transport
positive regulation of endothelial cell migration
cell differentiation
platelet activation
regulation of cell migration
negative regulation of cell migration
positive regulation of apoptotic process
positive regulation of angiogenesis
negative regulation of cell cycle
regulation of small GTPase mediated signal transduction
endothelial tube morphogenesis
cellular response to hydrogen peroxide
cellular response to ionizing radiation
