Invitrogen

S100A8 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3个已发表图片

2 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

S100A8 Antibody in Immunocytochemistry (ICC/IF)

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S100A8 Antibody (PA5-79948) in ICC/IF

Immunocytochemistry analysis of S100A8 using anti-S100A8 antibody (Product # PA5-79948). S100A8 was detected in a section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-S100A8 antibody (Product # PA5-79948) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visu... View More

S100A8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

S100A8 Antibody in Flow Cytometry (Flow)

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产品信息

PA5-79948

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.01mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2747063

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Salmonella pathogenicity island 1 knockdown confers protection against myocardial fibrosis and inflammation in uremic cardiomyopathy via down-regulation of S100 Calcium Binding Protein A8/A9 transcription.

Renal failure

Cai X,Hong L,Liu Y,Huang X,Lai H,Shao L

PA5-79948 was used in Western Blotting to dissect the mechanism of SPI1 in myocardial fibrosis and inflammation induced by UCM through S100A8/A9.

Thu Dec 01 00:00:00 EST 2022

Renal failure

Cai X,Hong L,Liu Y,Huang X,Lai H,Shao L

PA5-79948 was used in Western Blotting to dissect the mechanism of SPI1 in myocardial fibrosis and inflammation induced by UCM through S100A8/A9.

Thu Dec 01 00:00:00 EST 2022

S100A9 is a functional effector of infarct wall thinning after myocardial infarction.

American journal of physiology. Heart and circulatory physiology

Chalise U,Becirovic-Agic M,Daseke MJ,Konfrst SR,Rodriguez-Paar JR,Feng D,Salomon JD,Anderson DR,Cook LM,Lindsey ML

PA5-79948 was used in Western Blotting to determine protein changes over the course of neutrophil infiltration of ventricles following myocardial infarction in mice.

Tue Feb 01 00:00:00 EST 2022

生物信息学

蛋白别名: Calgranulin-A; Chemotactic cytokine CP-10; chemotactic S100 protein; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A)

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基因别名: 60B8Ag; AI323541; B8Ag; Caga; CFAg; CP-10; MRP8; p8; S100a8

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UniProt ID: (Rat) P50115, (Mouse) P27005

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Entrez Gene ID: (Rat) 116547, (Mouse) 20201

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功能

molecular_function calcium ion binding microtubule binding zinc ion binding antioxidant activity Toll-like receptor 4 binding arachidonic acid binding RAGE receptor binding metal ion binding calmodulin-related

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