Invitrogen

SALL4 Monoclonal Antibody (A7A6)

SALL4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

SALL4 Antibody (MA5-44967) in ICC/IF

Immunocytochemistry analysis of Sall4 in NCC-IT cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Samples were incubated in Sall4 Monoclonal antibody (Product # MA5-44967) using a dilution of 1:100 in 2% BSA overnight at 4 followed by Goat Anti-Mouse IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nuc... View More

SALL4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

产品信息

MA5-44967

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:600

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:500-1:10,000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

A7A6

抗原

Recombinant protein within human Sall4 aa 903-1,053/1,053

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931423

靶标信息

Sall4 protein is encoded by the Sall4 gene located on chromosome 20 in humans and belongs to the Spalt-like protein family. It plays an important role in somatic stem cell maintenance and is studied as a stem cell biomarker. It is a zinc finger transcription factor that is upregulated during development and shown to interact with Oct4 and Sox2. It also forms a complex with Nanog to maintain the pluripotency of Embryonic Stem cells (ESCs). Several current research studies focus on its function as an oncogene and tumor biomarker. Some of the reported target genes of Sall4 include p53, Bmi-1, Bcl2 and XIAP.

仅用于科研。不用于诊断过程。未经明确授权不得转售。