Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 3
SCF Antibody (500-P48GBT-1MG) in WB
Western Blot: To detect hSCF by Western Blot analysis SCF Polyclonal Antibody, Biotin (Product # 500-P48GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P48GBT-1MG
产品规格
宿主/亚型
Goat
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived, 18.4 kDa Recombinant Human SCF
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930578
产品详细信息
AA Sequence of recombinant protein: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human SCF. Anti-Human SCF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hSCF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SCF (500-P48G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hSCF.
Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P48GBT-1MG will be provided as 2 x 500 µg
靶标信息
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
