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SCF Antibody (500-P71-1MG) in WB
Western Blot: To detect Murine SCF by Western Blot analysis SCF Polyclonal Antibody (Product # 500-P71-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine SCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P71-1MG
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine SCF
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930714
产品详细信息
AA Sequence of recombinant protein: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine SCF. Anti-Murine SCF-specific antibody was purified by affinity chromatography employing an immobilized Murine SCF matrix.
Sandwich ELISA: To detect Murine SCF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine SCF (500-P71Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine SCF.
Western Blot: To detect Murine SCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine SCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-kit ligand; cloud gray; DKFZp686F2250; grizzle-belly; H-SCF; Hematopoietic growth factor KL; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; SCF; Steel factor; Stem cell factor
基因别名: blz; Clo; Con; contrasted; Gb; Kitl; Kitlg; Mgf; SCF; SF; Sl; SLF
UniProt ID: (Mouse) P20826
Entrez Gene ID: (Mouse) 17311
ovarian follicle development
neural crest cell migration
positive regulation of leukocyte migration
positive regulation of myeloid leukocyte differentiation
cell adhesion
germ cell development
positive regulation of cell proliferation
negative regulation of mast cell apoptotic process
embryonic hemopoiesis
ectopic germ cell programmed cell death
negative regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of melanocyte differentiation
positive regulation of DNA replication
positive regulation of Ras protein signal transduction
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of mast cell proliferation
extrinsic apoptotic signaling pathway in absence of ligand
