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SEC61B Antibody (PA3-015) in ICC/IF
Immunofluorescence analysis of SEC61B was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SEC61B Rabbit Polyclonal Antibody (Product # PA3-015) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
产品信息
PA3-015
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human,
Mouse,
Pig,
Rat
已发表种属
Human,
Pig
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues M(1) P G P T P S G T N V G S S G R S P S K(20) of canine sec61 beta.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
whole serum
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2239072
产品详细信息
PA3-015 detects human, canine and porcine, and rat sec61 beta from microsomes and shows no cross-reactivity with other sec61 isoforms.
PA3-015 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~14 kDa protein representing sec61 beta from pig microsomes.
The PA3-015 immunogen is a synthetic peptide corresponding to residues M(1) P G P T P S G T N V G S S G R S P S K(20) of canine sec61 beta. This sequence is completely conserved between human, mouse, and canine.
靶标信息
The Sec61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. Sec61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. Sec61 alpha is a polytopic protein that has ten membrane-spanning loops. Sec61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: protein translocation complex beta; protein translocation complex beta subunit; protein transport protein SEC61 beta subunit; Protein transport protein Sec61 subunit beta; sec61 beta; Sec61 beta subunit; Sec61 complex, beta subunit; SEC61 translocon beta subunit
基因别名: 1190006C12Rik; AI326121; AW122942; SEC61B
UniProt ID: (Human) P60468, (Dog) P60467, (Mouse) Q9CQS8
Entrez Gene ID: (Human) 10952, (Dog) 404018, (Pig) 100511362, (Mouse) 66212, (Rat) 298068
