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In Western Blot, a band is seen at ~50 kDa, representing the secreted form of the protein and also a faint band at ~83 kDa, representing the pro-form of the protein.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Suggested positive control: antigen standard for SEMA3B (transient overexpression lysate), mouse Cerebellum protein, mouse E16 cerebellum lysate.
The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Seam V antibody; sema A; Sema A(V); sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B; Sema V; semaphorin A; Semaphorin-3B; semaphorin-A; Semaphorin-V
基因别名: LUCA-1; SemA; SEMA3B; SEMA5; SEMAA; semaV
UniProt ID: (Human) Q13214
Entrez Gene ID: (Human) 7869, (Bovine) 539996, (Dog) 484762, (Mouse) 20347, (Rat) 363142