Invitrogen

SEMA3C Polyclonal Antibody

查看其他6个SEMA3C抗体

抗体检测数据 (2)

SEMA3C Antibody in Immunocytochemistry (ICC/IF)

SEMA3C Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

SEMA3C Antibody (PA5-103168) in ICC/IF

Immunocytochemistry analysis of SEMA3C in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SEMA3C polyclonal antibody (Product # PA5-103168) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

SEMA3C Antibody in Immunocytochemistry (ICC/IF)

SEMA3C Antibody in Western Blot (WB)

Product Image Gallery

SEMA3C Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of SEMA3C in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SEMA3C polyclonal antibody (Product # PA5-103168) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

SEMA3C Antibody in Western Blot (WB) — Western blot analysis of SEMA3C extracts from EC304 cells (heat-shock treatment). Samples were incubated with SEMA3C polyclonal antibody (Product # PA5-103168) using a dilution of 1:1,000. Observed bands: 70 kDa.

产品信息

PA5-103168

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SEMA3C(Accession Q99985), corresponding to amino acid residues H716-S751.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2852538

产品详细信息

Antibody detects endogenous levels of total SEMA3C.

靶标信息

SEMA3C may be involved in diverse cell survival mechanisms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C; Sema E; semaphorin E; Semaphorin-3C; Semaphorin-E

查看更多收起

基因别名: 1110036B02Rik; SEMA3C; SEMAE; SemE

查看更多收起

UniProt ID: (Human) Q99985, (Mouse) Q62181

查看更多收起

Entrez Gene ID: (Human) 10512, (Mouse) 20348, (Rat) 296787

查看更多收起

semaphorin receptor binding neuropilin binding chemorepellent activity receptor activity membrane-bound signaling molecule intercellular signal molecule

参与通路

neural crest cell migration somitogenesis blood vessel remodeling outflow tract morphogenesis cardiac right ventricle morphogenesis pulmonary myocardium development immune response axon guidance post-embryonic development neural tube development positive regulation of cell migration response to drug negative regulation of axon extension involved in axon guidance negative chemotaxis limb bud formation dichotomous subdivision of terminal units involved in salivary gland branching semaphorin-plexin signaling pathway multicellular organism development nervous system development heart development cell differentiation