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SEMA4F Antibody (PA5-87894) in IHC (P)
Immunohistochemistry analysis of SEMA4F in paraffin-embedded human esophageal cancer. Samples were incubated with SEMA4F Polyclonal antibody (Product # PA5-87894) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
产品信息
PA5-87894
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 280-420 of human SEMA4F (NP_0042542)
偶联物
形式
Liquid
浓度
3.3 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804486
产品详细信息
Immunogen sequence: RKTLQQRWTT FLKADLLCPG PEHGRASSVL QDVAVLRPEL GAGTPIFYGI FSSQWEGATI SAVCAFRPQD IRTVLNGPFR ELKHDCNRGL PVVDNDVPQP RPGECITNNM KLRHFGSSLS LPDRVLTFIR DHPLMDRPVF P; Positive Samples: SH-SY5Y, HepG2, A-549, Mouse brain, Mouse liver, Mouse lung, Rat brain, Rat liver; Cellular Location: Membrane, Single-pass type I membrane protein
靶标信息
SEMA4F has growth cone collapse activity against retinal ganglion-cell axons.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: sema domain immunoglobulin domain transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4F; sema domain, immunoglobulin domain (Ig), TM domain, and short cy; sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F; Sema M; Sema W; semaphorin 4f; semaphorin M; Semaphorin-4F; Semaphorin-M; Semaphorin-W
基因别名: M-SEMA; m-Sema-M; PRO2353; S4F; Sema W; SEMA4F; SEMAM; SEMAW
UniProt ID: (Human) O95754, (Rat) Q9Z143, (Mouse) Q9Z123
Entrez Gene ID: (Human) 10505, (Rat) 29745, (Mouse) 20355
neural crest cell migration
cell-cell signaling
nervous system development
axon guidance
positive regulation of cell migration
retinal ganglion cell axon guidance
negative regulation of axon extension involved in axon guidance
negative chemotaxis
semaphorin-plexin signaling pathway
negative regulation of axon extension
