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SETDB1 Antibody (MA5-14960) in ICC/IF
Immunofluorescence analysis of SETDB1 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SETDB1 Monoclonal Antibody (K.137.7) (Product # MA5-14960) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (P... View More
产品信息
MA5-14960
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Non-human primate
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
K.137.7
抗原
Synthetic peptide corresponding to the carboxy terminus of the human ESET protein
偶联物
形式
Liquid
浓度
9 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_11004449
产品详细信息
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other SET-domain containing histone methyltransferase proteins.
靶标信息
This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ERG-associated protein with a SET domain, ESET; ERG-associated protein with SET domain; ESET; H3-K9-HMTase 4; Histone H3-K9 methyltransferase 4; Histone-lysine N-methyltransferase SETDB1; histone-lysine N-methyltransferase, H3lysine-9 specific 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SET domain, bifurcated 1; tudor domain containing 21
基因别名: ESET; H3-K9-HMTase4; KG1T; KIAA0067; KMT1E; SETDB1; TDRD21
UniProt ID: (Human) Q15047
Entrez Gene ID: (Human) 9869
