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SF1 Antibody (MA5-27159) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a SF1 monoclonal antibody (Product # MA5-27159) at a dilution of 1:150.
产品信息
MA5-27159
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
OTI7H9
抗原
Human recombinant protein fragment corresponding to amino acids 1-276 of SF1 produced in E.coli
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 1% BSA, 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2725336
靶标信息
Splicing factor 1 is a nuclear pre-mRNA splicing factor. The protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BBP; M ZFM1; Mammalian branch point-binding protein; Splicing factor 1; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162
基因别名: BBP; D11S636; MBBP; SF1; ZCCHC25; ZFM1; ZNF162
UniProt ID: (Human) Q15637
Entrez Gene ID: (Human) 7536
spliceosomal complex assembly
mRNA 3'-splice site recognition
mRNA splicing, via spliceosome
transcription, DNA-templated
regulation of transcription, DNA-templated
male sex determination
Leydig cell differentiation
negative regulation of smooth muscle cell proliferation
regulation of steroid biosynthetic process
negative regulation of nucleic acid-templated transcription
