Proteintech

SFRP2 Monoclonal Antibody (1F3A1)

1 篇参考文献

查看其他10个SFRP2抗体

抗体检测数据 (6)

SFRP2 Antibody in Immunocytochemistry (ICC/IF)

SFRP2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

SFRP2 Antibody (66328-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 66328-1-IG (SFRP2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

SFRP2 Antibody in Immunocytochemistry (ICC/IF)

SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SFRP2 Antibody in Western Blot (WB)

SFRP2 Antibody in Western Blot (WB)

SFRP2 Antibody in Western Blot (WB)

Product Image Gallery

SFRP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 66328-1-IG (SFRP2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human malignant melanoma tissue slide using 66328-1-IG ( SFRP2 Antibody) at dilution of 1:200 (under 40x lens).

SFRP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human malignant melanoma tissue slide using 66328-1-IG ( SFRP2 Antibody) at dilution of 1:200 (under 10x lens).

SFRP2 Antibody in Western Blot (WB) — Rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-IG ( SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SFRP2 Antibody in Western Blot (WB) — COLO 320 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

SFRP2 Antibody in Western Blot (WB) — Y79 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

产品信息

66328-1-IG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:500

-

免疫细胞化学 (ICC/IF)

1:50-1:500

-

产品规格

种属反应

Human, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1F3A1

抗原

SFRP2 Fusion Protein Ag18840 (1-295 aa encoded by BC008666)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: MLQGPGSLL LLFLASHCCL GSARGLFLFG QPDFSYKRSN CKPIPVNLQL CHGIEYQNMR LPNLLGHETM KEVLEQAGAW IPLVMKQCHP DTKKFLCSLF APVCLDDLDE TIQPCHSLCV QVKDRCAPVM SAFGFPWPDM LECDRFPQDN DLCIPLASSD HLLPATEEAP KVCEACKNKN DDDNDIMETL CKNDFALKIK VKEITYINRD TKIILETKSK TIYKLNGVSE RDLKKSVLWL KDSLQCTCEE MNDINAPYLV MGQKQGGELV ITSVKRWQKG QREFKRISRS IRKLQC (1-295 aa encoded by B C008666)

靶标信息

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

miR-629-5p promotes growth and metastasis of hepatocellular carcinoma by activating β-catenin.

Experimental cell research

Tao X,Yang X,Wu K,Yang L,Huang Y,Jin Q,Chen S

Mon Jul 15 00:00:00 EDT 2019

生物信息学

蛋白别名: FRP 2; FRP-2; MGC128911; MGC153618; MGC53344; SARP 1; SARP-1; SDF 5; secreted apoptosis related protein 1; Secreted apoptosis-related protein 1; Secreted frizzled-related protein 2; sFRP 2; sFRP-2; Sfrp2 protein; testicular tissue protein Li 170

查看更多收起

基因别名: FKSG12; FRP-2; FRP2; SARP1; SDF-5; SFRP2; UNQ361/PRO697

查看更多收起

UniProt ID: (Human) Q96HF1

查看更多收起

Entrez Gene ID: (Human) 6423, (Rat) 310552

查看更多收起

fibronectin binding G-protein coupled receptor activity integrin binding Wnt-protein binding Wnt-activated receptor activity receptor agonist activity endopeptidase activator activity molecular_function enzyme activator activity transmembrane signal receptor

参与通路

patterning of blood vessels chondrocyte development outflow tract morphogenesis cardiac left ventricle morphogenesis apoptotic process G-protein coupled receptor signaling pathway cell-cell signaling response to nutrient positive regulation of cell proliferation negative regulation of cell proliferation male gonad development negative regulation of gene expression negative regulation of cardiac muscle cell apoptotic process negative regulation of epithelial to mesenchymal transition positive regulation of endopeptidase activity negative regulation of Wnt signaling pathway collagen fibril organization positive regulation of cell growth negative regulation of cell growth negative regulation of cell migration negative regulation of BMP signaling pathway cellular response to extracellular stimulus positive regulation of peptidyl-serine phosphorylation positive regulation of cell adhesion mediated by integrin positive regulation of catenin import into nucleus non-canonical Wnt signaling pathway post-anal tail morphogenesis response to drug negative regulation of mesodermal cell fate specification embryonic digit morphogenesis positive regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of JUN kinase activity positive regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of angiogenesis negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter digestive tract morphogenesis negative regulation of epithelial cell proliferation negative regulation of peptidyl-tyrosine phosphorylation convergent extension involved in axis elongation bone morphogenesis sclerotome development negative regulation of dermatome development hematopoietic stem cell proliferation cellular response to X-ray negative regulation of canonical Wnt signaling pathway planar cell polarity pathway involved in neural tube closure Wnt signaling pathway involved in somitogenesis positive regulation of canonical Wnt signaling pathway negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage regulation of dopaminergic neuron differentiation midbrain morphogenesis regulation of stem cell division negative regulation of planar cell polarity pathway involved in axis elongation somitogenesis neural tube closure signal transduction anterior/posterior pattern specification neural tube development regulation of Wnt signaling pathway regulation of cell proliferation regulation of apoptotic process development of primary male sexual characteristics cartilage development regulation of establishment of planar polarity positive regulation of receptor activity