Invitrogen

SHH Polyclonal Antibody

SHH Antibody in Immunocytochemistry (ICC/IF)

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SHH Antibody (PA5-142192) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of SHH in NIH3T3 cells using SHH Polyclonal Antibody (Product # PA5-142192). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

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产品信息

PA5-142192

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

免疫细胞化学 (ICC/IF)

10 µg/mL

流式细胞分析 (Flow)

10 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide with sequence C-DSEALHPLGMAVK (aa 448-460).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2932848

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.

靶标信息

Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: hemimelic extra toes; HHG-1; SHH; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; short digits; sonic hedgehog homolog; Sonic hedgehog protein

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基因别名: 9530036O11Rik; Dsh; HHG1; HLP3; HPE3; Hx; Hxl3; M100081; MCOPCB5; SHH; SMMCI; TPT; TPTPS

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UniProt ID: (Human) Q15465, (Mouse) Q62226

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Entrez Gene ID: (Human) 6469, (Mouse) 20423

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