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SHP-1 Antibody (PA5-118222) in WB
Western Blot using SHP-1 Polyclonal Antibody (Product # PA5-118222) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: k562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 68 kDa. Observed band size: 68 kDa. (We are unsure of the identity of these extra bands).
产品信息
PA5-118222
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Mouse PTPN6 Protein
偶联物
形式
Liquid
纯化类型
Antigen affinity chromatography, Protein A
保存液
PBS
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2902822
靶标信息
SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 70Z-SHP; EC 3.1.3.48; Hematopoietic cell protein-tyrosine phosphatase; motheaten; Protein-tyrosine phosphatase 1C; PTN6; PTP-1C; PTPTY-42; SH-PTP1; SHP-1; Tyrosine-protein phosphatase non-receptor type 6
基因别名: 70Z-SHP; Hcp; Hcph; me; Ptp1C; Ptpn6; PTPTY-42; SH-PTP1; SHP-1
UniProt ID: (Mouse) P29351
Entrez Gene ID: (Mouse) 15170
phosphotyrosine binding
phosphoprotein phosphatase activity
protein tyrosine phosphatase activity
transmembrane receptor protein tyrosine phosphatase activity
protein binding
hydrolase activity
phosphatase activity
SH3 domain binding
protein kinase binding
SH2 domain binding
natural killer cell lectin-like receptor binding
protein phosphatase
hematopoietic progenitor cell differentiation
negative regulation of humoral immune response mediated by circulating immunoglobulin
protein dephosphorylation
signal transduction
cell proliferation
positive regulation of cell proliferation
positive regulation of phosphatidylinositol 3-kinase signaling
dephosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
cell differentiation
platelet formation
abortive mitotic cell cycle
positive regulation of cell adhesion mediated by integrin
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
megakaryocyte development
negative regulation of T cell proliferation
natural killer cell mediated cytotoxicity
negative regulation of MAP kinase activity
negative regulation of MAPK cascade
regulation of B cell differentiation
negative regulation of peptidyl-tyrosine phosphorylation
B cell receptor signaling pathway
negative regulation of B cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
regulation of release of sequestered calcium ion into cytosol
regulation of ERK1 and ERK2 cascade
platelet aggregation
regulation of G1/S transition of mitotic cell cycle
