Invitrogen

SHP-1 Monoclonal Antibody (R.488.4)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
高级验证 (1)

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SHP-1 Antibody (MA5-14839) in WB

Western blot was performed using Anti-SHP-1 Monoclonal Antibody (R.488.4)(Product # MA5-14839) and a 63 kDa band corresponding to SHP-1 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), THP-1 (Lane 2), HEL 92.1.7 (Lane 3), K-562 (Lane 4), U-2 OS (Lane 5), Mouse Spleen (Lane 6), Rat Spleen (Lane 7), Mouse Skeletal Muscle (Lane 8), Rat Skeletal Muscle (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred... View More

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SHP-1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissue tested owing to their inherent genetic constitution. Relative expression of SHP-1 was observed in Jurkat, THP-1, HEL.92.1.7, MCF-7 and Mouse Spleen and Rat spleen, which are high expressing models, and was found to be negative in K-562, U-2 OS, Mouse Skeletal muscle and Rat Skeletal muscle, which are low expressing models as reported. using Anti-SHP-1 Monoclonal Antibody (R.488.4) (Product # MA5-14839) in Western Blot. {RE}

产品信息

MA5-14839

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

R.488.4

抗原

Synthetic peptide corresponding to residues surrounding Pro161 of human SHP-1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

92 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10983151

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 70Z-SHP; EC 3.1.3.48; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; motheaten; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; PTPTY-42; SH-PTP1; SH2 phosphatase 1; SHP-1; Tyrosine-protein phosphatase non-receptor type 6

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基因别名: 70Z-SHP; HCP; HCPH; HPTP1C; me; PTP-1C; PTP1C; Ptph6; PTPN6; PTPTY-42; SH-PTP1; SHP-1; SHP-1L; SHP1

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UniProt ID: (Human) P29350, (Rat) P81718, (Mouse) P29351

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Entrez Gene ID: (Human) 5777, (Rat) 116689, (Mouse) 15170

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