Invitrogen

SIAH2 Polyclonal Antibody

查看其他7个SIAH2抗体

抗体检测数据 (1)

SIAH2 Antibody in Western Blot (WB)

SIAH2 Antibody in Western Blot (WB)

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SIAH2 Antibody (PA5-102907) in WB

Western blot analysis of SIAH2 in NIH-3T3 cell lysate. Samples were incubated with SIAH2 polyclonal antibody (Product # PA5-102907).

SIAH2 Antibody in Western Blot (WB)

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SIAH2 Antibody in Western Blot (WB) — Western blot analysis of SIAH2 in NIH-3T3 cell lysate. Samples were incubated with SIAH2 polyclonal antibody (Product # PA5-102907).

产品信息

PA5-102907

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SIAH2(Accession O43255), corresponding to amino acid residues F246-C296.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2852288

产品详细信息

Antibody detects endogenous levels of total SIAH2.

靶标信息

SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1 alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: E3 ubiquitin-protein ligase SIAH2; EC 6.3.2; mSiah2; RING-type E3 ubiquitin transferase SIAH2; Seven in absentia homolog 2; Siah-2

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基因别名: AA415433; hSiah2; SIAH2; Sinh2

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UniProt ID: (Human) O43255, (Mouse) Q06986

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Entrez Gene ID: (Human) 6478, (Mouse) 20439

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transcription corepressor activity ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity ubiquitin conjugating enzyme binding ubiquitin protein ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase protein modifying enzyme

参与通路

protein polyubiquitination ubiquitin-dependent protein catabolic process apoptotic process cell cycle small GTPase mediated signal transduction axon guidance regulation of protein ubiquitination protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process cellular protein catabolic process cellular protein metabolic process negative regulation of canonical Wnt signaling pathway negative regulation of nucleic acid-templated transcription negative regulation of extrinsic apoptotic signaling pathway multicellular organism development protein ubiquitination