Invitrogen

SIN3A Polyclonal Antibody

SIN3A Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

SIN3A Antibody (PA1-870) in ICC/IF

Immunofluorescent analysis of Sin3A (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sin3A polyclonal antibody (Product # PA1-870) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated ... View More

SIN3A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

产品信息

PA1-870

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:500

免疫细胞化学 (ICC/IF)

1:50-1:500

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent

染色质免疫沉淀实验 (ChIP)

1-3 µL

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2187625

产品详细信息

PA1-870 detects mSin3A from human and mouse tissues and cells.

PA1-870 has been successfully used in Western blot, ICC/IF ChIP, IHC-P and immunoprecipitation procedures. By Western blot, this antibody detects an ~150 kDa protein representing mSin3A from T47D cell extract.

PA1-870 immunogen is a synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A.

靶标信息

Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

An Epigenetic Mechanism Underlying Chromosome 17p Deletion-Driven Tumorigenesis.

Cancer discovery

Chen M,Chen X,Li S,Pan X,Gong Y,Zheng J,Xu J,Zhao C,Zhang Q,Zhang S,Qi L,Wang Z,Shi K,Ding BS,Xue Z,Chen L,Yang S,Wang Y,Niu T,Dai L,Lowe SW,Chen C,Liu Y

PA1-870 was used in Western Blot to identify a new 17p tumor suppressor gene (TSG), plant homeodomain finger protein 23 (PHF23).

Fri Jan 01 00:00:00 EST 2021

An Epigenetic Mechanism Underlying Chromosome 17p Deletion-Driven Tumorigenesis.

Cancer discovery

Chen M,Chen X,Li S,Pan X,Gong Y,Zheng J,Xu J,Zhao C,Zhang Q,Zhang S,Qi L,Wang Z,Shi K,Ding BS,Xue Z,Chen L,Yang S,Wang Y,Niu T,Dai L,Lowe SW,Chen C,Liu Y

PA1-870 was used in Western Blot to identify a new 17p tumor suppressor gene (TSG), plant homeodomain finger protein 23 (PHF23).

Fri Jan 01 00:00:00 EST 2021

Biotin tagging of MeCP2 in mice reveals contextual insights into the Rett syndrome transcriptome.

Nature medicine

Johnson BS,Zhao YT,Fasolino M,Lamonica JM,Kim YJ,Georgakilas G,Wood KH,Bu D,Cui Y,Goffin D,Vahedi G,Kim TH,Zhou Z

PA1-870 was used in Western Blotting to develop a Cre-inducible method for cell type-specific biotin tagging of MeCP2 in mice for studying the Rett syndrome transcriptome.

Sun Oct 01 00:00:00 EDT 2017

Elevating expression of MeCP2 T158M rescues DNA binding and Rett syndrome-like phenotypes.

The Journal of clinical investigation

Lamonica JM,Kwon DY,Goffin D,Fenik P,Johnson BS,Cui Y,Guo H,Veasey S,Zhou Z

Published figure using SIN3A polyclonal antibody (Product # PA1-870) in Western Blot

Mon May 01 00:00:00 EDT 2017

RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing.

Nucleic acids research

Khan DH,Gonzalez C,Cooper C,Sun JM,Chen HY,Healy S,Xu W,Smith KT,Workman JL,Leygue E,Davie JR

PA1-870 was used in western blot to study the modulation of MCL1 alternative splicing by an RNA-dependent mechanism involving dynamic histone acetylation

Sat Feb 01 00:00:00 EST 2014

Dynamic distribution of HDAC1 and HDAC2 during mitosis: association with F-actin.

Journal of cellular physiology

He S,Khan DH,Winter S,Seiser C,Davie JR

PA1-870 was used in immunocytochemistry to study the mitotic dynamics of HDAC-1 and -2 and their association with F-actin

Mon Jul 01 00:00:00 EDT 2013

Protein kinase CK2 regulates the dimerization of histone deacetylase 1 (HDAC1) and HDAC2 during mitosis.

The Journal of biological chemistry

Khan DH,He S,Yu J,Winter S,Cao W,Seiser C,Davie JR

PA1-870 was used in western blot to study the modulation of the dimerization of HDAC-1 and -2 by CK2-mediated phosphorylation during mitosis

Fri Jun 07 00:00:00 EDT 2013

Differential requirement of histone acetylase and deacetylase activities for IRF5-mediated proinflammatory cytokine expression.

Journal of immunology (Baltimore, Md. : 1950)

Feng D,Sangster-Guity N,Stone R,Korczeniewska J,Mancl ME,Fitzgerald-Bocarsly P,Barnes BJ

PA1-870 was used in immunoprecipitation and western blot to investigate the regulation of IRF5-mediated proinflammatory cytokine expression

Mon Nov 15 00:00:00 EST 2010

生物信息学

蛋白别名: Histone deacetylase complex subunit Sin3a; Mammalian Sin3A; Paired amphipathic helix protein Sin3a; SIN; SIN3 homolog A, transcription regulator; SIN3 transcription regulator homolog A; SIN3A; transcriptional co-repressor Sin3A; Transcriptional corepressor Sin3a; transcriptional regulator, SIN3 yeast homolog A; transcriptional regulator, SIN3A

查看更多收起

基因别名: AW553200; Kiaa4126; mKIAA4126; mSin3A; Sin3; SIN3A

查看更多收起

UniProt ID: (Human) Q96ST3, (Mouse) Q60520

查看更多收起

Entrez Gene ID: (Human) 25942, (Mouse) 20466

查看更多收起

功能

transcription regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding RNA polymerase II repressing transcription factor binding RNA polymerase II transcription corepressor activity chromatin binding transcription factor activity, sequence-specific DNA binding RNA binding histone deacetylase activity protein binding protein complex binding protein deacetylase activity DNA binding transcription corepressor activity transcription factor binding chromatin/chromatin-binding, or -regulatory protein

参与通路

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development activation of innate immune response positive regulation of defense response to virus by host hematopoietic progenitor cell differentiation DNA replication transcription, DNA-templated protein deacetylation aging regulation of hormone levels positive regulation of G2/M transition of mitotic cell cycle histone deacetylation positive regulation of chromatin silencing cellular protein localization negative regulation of circadian rhythm negative regulation of apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress cellular lipid metabolic process negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process response to methylglyoxal cellular response to glucose stimulus negative regulation of protein localization to nucleus negative regulation of histone H3-K27 acetylation cellular response to dopamine negative regulation of transcription regulatory region DNA binding regulation of transcription, DNA-templated transcription from RNA polymerase II promoter response to organonitrogen compound

SIN3A Polyclonal Antibody

SIN3A Antibody (PA1-870) in ICC/IF

产品信息

PA1-870

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

SIN3A Polyclonal Antibody

SIN3A Antibody (PA1-870) in ICC/IF

产品信息

PA1-870

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)