Invitrogen

SIRT3 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1个已发表图片

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

SIRT3 Antibody in Immunocytochemistry (ICC/IF)

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SIRT3 Antibody (PA5-96406) in ICC/IF

Immunofluorescence analysis of NAD-dependent protein deacetylase sirtuin-3, mitochondrial was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SIRT3 Polyclonal Antibody (Product # PA5-96406) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secon... View More

SIRT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SIRT3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with NAD-dependent protein deacetylase sirtuin-3, mitochondrial siRNA and decrease in signal intensity was observed in Western Blot application using Anti-SIRT3 Polyclonal Antibody (Product # PA5-96406). {KD}

产品信息

PA5-96406

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫组化（石蜡） (IHC (P))

1:20-1:50

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 299-399 of human SIRT3 (NP_0363711)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2808208

产品详细信息

Positive Samples: SW620; Cellular Location: Mitochondrion matrix

Immunogen sequence: PQRFLLHVVD FPMADLLLIL GTSLEVEPFA SLTEAVRSSV PRLLINRDLV GPLAWHPRSR DVAQLGDVVH GVESLVELLG WTEEMRDLVQ RETGKLDGPD K

靶标信息

SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats.

Redox biology

Li M,Yu Y,Xue K,Li J,Son G,Wang J,Qian W,Wang S,Zheng J,Yang C,Ge J

Published figure using SIRT3 polyclonal antibody (Product # PA5-96406) in Western Blot

Mon May 01 00:00:00 EDT 2023

生物信息学

蛋白别名: HGNC:14931; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; mitochondrial protein lysine deacetylase; mSIR2L3; NAD-dependent deacetylase sirtuin-3; NAD-dependent deacetylase sirtuin-3, mitochondrial; NAD-dependent protein deacetylase sirtuin-3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; SIRT3L mitochondrial; sirtuin type 3

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基因别名: 2310003L23Rik; AI848213; SIR2L3; SIRT3

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UniProt ID: (Human) Q9NTG7, (Mouse) Q8R104

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Entrez Gene ID: (Human) 23410, (Mouse) 64384, (Rat) 293615

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功能