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SKA3 Antibody (A304-214A) in IP
Detection of human SKA3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SKA3 antibody A304-214A (lot A304-214A-1) used for IP at 6 µg/mg lysate. SKA3 was also immunoprecipitated by rabbit anti-SKA3 antibody A304-215A. For blotting immunoprecipitated SKA3, A304-215A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
产品信息
A304-214A
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 175 to 225 of Human Spindle and Kinetochore Associated Complex Subunit 3.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
phosphate/tris citrate, pH 7-8
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
靶标信息
Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: RP11-101P17.12-002; Spindle and kinetochore-associated protein 3
基因别名: C13orf3; RAMA1; SKA3
UniProt ID: (Human) Q8IX90
Entrez Gene ID: (Human) 221150
