Invitrogen

SOCS3 Monoclonal Antibody (SO1)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 篇参考文献

查看其他17个SOCS3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

SOCS3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

SOCS3 Antibody (MA1-19373) in ICC/IF

Immunofluorescence analysis of SOCS3 was performed using 70% confluent log phase HT-29 cells treated with 10 ng of IL-6 for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SOCS3 Mouse Monoclonal Antibody (Product # MA1-19373) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor®... View More

Product Image Gallery

SOCS3 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of SOCS3 using SOCS3 Monoclonal Antibody (Product # MA1-19373) shows increased expression of proteins in A-375 cell line upon IFN gamma treatment and in HT-29 cell line upon IL-6 treatment. {TM}

产品信息

MA1-19373

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

SO1

抗原

Full-length SOCS3 protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1087394

产品详细信息

This antibody reacts with SOCS3, an intracellular cytokine signaling inhibitor.

Western Blot: Reducing conditions.

靶标信息

Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways.

仅用于科研。不用于诊断过程。未经明确授权不得转售。