Invitrogen

SP1 Polyclonal Antibody

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SP1 Antibody in Western Blot (WB)

SP1 Antibody in Western Blot (WB)

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SP1 Antibody (PA5-27243) in WB

Western Blot using SP1 Polyclonal Antibody (Product # PA5-27243). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2. 5% SDS PAGE. SP1 Polyclonal Antibody (Product # PA5-27243) diluted at 1:1,000.

SP1 Antibody in Western Blot (WB)

SP1 Antibody in Immunocytochemistry (ICC/IF)

SP1 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

SP1 Antibody in Western Blot (WB) — Western Blot using SP1 Polyclonal Antibody (Product # PA5-27243). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2. 5% SDS PAGE. SP1 Polyclonal Antibody (Product # PA5-27243) diluted at 1:1,000.

SP1 Antibody in Immunocytochemistry (ICC/IF) — Figure 1 Localization and expression of thiamine transporters and Sp1 inside renal glomerular cells in physiological conditions. ( a ) Immunofluorescence with Ab anti-THTR1, anti-THTR2, and anti-Sp1 in HPTEC, HPC, HGEC, and HMC (green). Nuclei are counterstained with DAPI (blue). Magnification 400x. ( b ) Relative expression of THTR-1, THTR2, and Sp1 in the 4 cell types. Mean of 5 experiments +- SD.

SP1 Antibody in Immunocytochemistry (ICC/IF) — Figure 1. Expression of thiamine transporters and Sp1 in retinal cells in physiological conditions: comparison of mRNA (a) and protein expression (b) in the different cell types. (c-k) Localization of thiamine transporters and Sp1 inside retinal cells in physiological conditions. Immunofluorescence with Ab (c, f, i) anti-THTR1, (d, g, j) anti-THTR2 and (e, h, k) anti-Sp1 in (c-e) HMEC, (f-h) HRP and (i-k) MIO-M1 cells (green). Nuclei are counterstained with DAPI (blue). Magnification 400x.

产品信息

PA5-27243

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫细胞化学 (ICC/IF)

-

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 470 and 564 of Human SP1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544719

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Mouse (100%), Rat (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Reduced Thiamine Availability and Hyperglycemia Impair Thiamine Transport in Renal Glomerular Cells through Modulation of Thiamine Transporter 2.

Biomedicines

Mazzeo A,Barutta F,Bellucci L,Trento M,Gruden G,Porta M,Beltramo E

Published figure using SP1 polyclonal antibody (Product # PA5-27243) in Immunocytochemistry

Mon Apr 05 00:00:00 EDT 2021

Reduced Thiamine Availability and Hyperglycemia Impair Thiamine Transport in Renal Glomerular Cells through Modulation of Thiamine Transporter 2.

Biomedicines

Mazzeo A,Barutta F,Bellucci L,Trento M,Gruden G,Porta M,Beltramo E

Published figure using SP1 polyclonal antibody (Product # PA5-27243) in Immunocytochemistry

Mon Apr 05 00:00:00 EDT 2021

Thiamine transporter 2 is involved in high glucose-induced damage and altered thiamine availability in cell models of diabetic retinopathy.

Diabetes & vascular disease research

Beltramo E,Mazzeo A,Lopatina T,Trento M,Porta M

Published figure using SP1 polyclonal antibody (Product # PA5-27243) in Immunocytochemistry

Tue Jun 30 00:00:00 EDT 2020

生物信息学

蛋白别名: HGNC:11205; specificity protein 1; Transcription factor Sp1

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基因别名: SP1; TSFP1

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UniProt ID: (Human) P08047

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Entrez Gene ID: (Human) 6667

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RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter sequence-specific DNA binding RNA polymerase II repressing transcription factor binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding transcription factor binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding HMG box domain binding C2H2 zinc finger transcription factor

参与通路

regulation of transcription, DNA-templated viral process snRNA transcription from RNA polymerase II promoter positive regulation by host of viral transcription positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process regulation of phenotypic switching by transcription from RNA polymerase II promoter positive regulation of hydrogen sulfide biosynthetic process