Invitrogen

SP1 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9个已发表图片

12 篇参考文献

查看其他35个SP1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

SP1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 27

SP1 Antibody (PA5-29165) in ICC/IF

Immunofluorescence analysis of SP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SP1 Polyclonal Antibody (Product # PA5-29165) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

SP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with SP1 siRNA and reduction of signal was observed in Western Blot using SP1 Polyclonal Antibody (Product # PA5-29165). {KD}

产品信息

PA5-29165

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看7篇已发表文章 7篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀实验 (ChIP)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

凝胶阻滞分析 (GS)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 18 and 303 of Human SP1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.22 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2546641

产品详细信息

Recommended positive controls: 293T, K562, THP-1, HL-60, Raji.

Predicted reactivity: Mouse (96%), Rat (99%), Rhesus Monkey (99%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

LncRNA NCAL1 potentiates natural killer cell cytotoxicity through the Gab2-PI3K-AKT pathway.

Frontiers in immunology

Niu C,Li M,Chen Y,Zhang X,Zhu S,Zhou X,Zhou L,Li Z,Xu J,Hu JF,Wang Y,Cui J

Published figure using SP1 polyclonal antibody (Product # PA5-29165) in ChIP Assay

Tue Oct 18 00:00:00 EDT 2022

An Arginine-Rich Motif in the ORF2 capsid protein regulates the hepatitis E virus lifecycle and interactions with the host cell.

PLoS pathogens

Hervouet K,Ferrié M,Ankavay M,Montpellier C,Camuzet C,Alexandre V,Dembélé A,Lecoeur C,Foe AT,Bouquet P,Hot D,Vausselin T,Saliou JM,Salomé-Desnoulez S,Vandeputte A,Marsollier L,Brodin P,Dreux M,Rouillé Y,Dubuisson J,Aliouat-Denis CM,Cocquerel L

PA5-29165 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that the ARM controls ORF2 nuclear translocation likely to promote regulation of host antiviral responses.

Mon Aug 01 00:00:00 EDT 2022

An Arginine-Rich Motif in the ORF2 capsid protein regulates the hepatitis E virus lifecycle and interactions with the host cell.

PLoS pathogens

Mon Aug 01 00:00:00 EDT 2022

High expression of GSKIP is associated with poor prognosis in meningioma.

Medicine

Cheng YW,Chen YY,Lin CJ,Lieu AS,Tsai HP,Kwan AL

PA5-29165 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that GSKIP may be a biomarker of poor prognosis and a target protein for therapy in meningioma.

Fri Dec 16 00:00:00 EST 2022

Treponema denticola dentilisin triggered TLR2/MyD88 activation upregulates a tissue destructive program involving MMPs via Sp1 in human oral cells.

PLoS pathogens

Ganther S,Radaic A,Malone E,Kamarajan P,Chang NN,Tafolla C,Zhan L,Fenno JC,Kapila YL

Published figure using SP1 polyclonal antibody (Product # PA5-29165) in Western Blot

Thu Jul 01 00:00:00 EDT 2021

Identification of PIM1 substrates reveals a role for NDRG1 phosphorylation in prostate cancer cellular migration and invasion.

Communications biology

Ledet RJ,Ruff SE,Wang Y,Nayak S,Schneider JA,Ueberheide B,Logan SK,Garabedian MJ

PA5-29165 was used in Western Blot to show that PIM1 phosphorylation of NDRG1 at S330 reduced its stability, nuclear localization, and interaction with AR, resulting in enhanced cell migration and invasion.

Mon Jan 04 00:00:00 EST 2021

Identification of PIM1 substrates reveals a role for NDRG1 phosphorylation in prostate cancer cellular migration and invasion.

Communications biology

Ledet RJ,Ruff SE,Wang Y,Nayak S,Schneider JA,Ueberheide B,Logan SK,Garabedian MJ

Mon Jan 04 00:00:00 EST 2021

Multi-layered epigenetic regulation of IRS2 expression in the liver of obese individuals with type 2 diabetes.

Diabetologia

Krause C,Geißler C,Tackenberg H,El Gammal AT,Wolter S,Spranger J,Mann O,Lehnert H,Kirchner H

PA5-29165 was used in Gel Shift to study a new multi-layered epigenetic network that could be involved in subtle dysregulation of IRS2 in the liver of individuals with type 2 diabetes.

Thu Oct 01 00:00:00 EDT 2020

Analysis of proximal ALOX5 promoter binding proteins by quantitative proteomics.

The FEBS journal

Schlag K,Steinhilber D,Karas M,Sorg BL

Published figure using SP1 polyclonal antibody (Product # PA5-29165) in Western Blot

Thu Oct 01 00:00:00 EDT 2020

Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion.

Cell death and differentiation

Li Y,Feng D,Wang Z,Zhao Y,Sun R,Tian D,Liu D,Zhang F,Ning S,Yao J,Tian X

PA5-29165 was used in Western Blotting to study the function and mechanism of ferroptosis in ischemia/reperfusion injury.

Fri Nov 01 00:00:00 EDT 2019

Specific protein 1, c-Abl and ERK1/2 form a regulatory loop.

Journal of cell science

Long J,Liao G,Wang Y,Tang DD

Published figure using SP1 polyclonal antibody (Product # PA5-29165) in Western Blot

Wed Jan 02 00:00:00 EST 2019

Decitabine Inhibits Gamma Delta T Cell Cytotoxicity by Promoting KIR2DL2/3 Expression.

Frontiers in immunology

Niu C,Li M,Zhu S,Chen Y,Zhou L,Xu D,Li W,Cui J,Liu Y,Chen J

PA5-29165 was used in Chromatin immunoprecipitation to deomstrate that decitabine inhibits the function of gamma delta T cells at both cellular and molecular levels.

Tue Jun 25 00:00:00 EDT 2019

Long interspersed nuclear element-1 expression and retrotransposition in prostate cancer cells.

Mobile DNA

Briggs EM,Ha S,Mita P,Brittingham G,Sciamanna I,Spadafora C,Logan SK

Published figure using SP1 polyclonal antibody (Product # PA5-29165) in Western Blot

Mon Nov 13 00:00:00 EST 2023

生物信息学

蛋白别名: HGNC:11205; specificity protein 1; Transcription factor Sp1

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基因别名: SP1; TSFP1

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UniProt ID: (Human) P08047

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Entrez Gene ID: (Human) 6667

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功能

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter sequence-specific DNA binding RNA polymerase II repressing transcription factor binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding transcription factor binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding HMG box domain binding C2H2 zinc finger transcription factor

参与通路

regulation of transcription, DNA-templated viral process snRNA transcription from RNA polymerase II promoter positive regulation by host of viral transcription positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process regulation of phenotypic switching by transcription from RNA polymerase II promoter positive regulation of hydrogen sulfide biosynthetic process

SP1 Polyclonal Antibody

SP1 Antibody (PA5-29165) in ICC/IF

产品信息

PA5-29165

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

凝胶阻滞分析 (GS)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

SP1 Polyclonal Antibody

SP1 Antibody (PA5-29165) in ICC/IF

产品信息

PA5-29165

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

凝胶阻滞分析 (GS)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)