Proteintech

SP1 Monoclonal Antibody (4G3H3)

2 篇参考文献

查看其他36个SP1抗体

抗体检测数据 (7)

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 7

SP1 Antibody (66508-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SP1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

SP1 Antibody in Western Blot (WB)

SP1 Antibody in Western Blot (WB)

Product Image Gallery

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66508-1-IG ( SP1 antibody) at dilution of 1:350 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SP1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using SP1 antibody (Product # 66508-1-IG, Clone: 4G3H3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

SP1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SP1 Antibody in Western Blot (WB) — Various cells were subjected to SDS PAGE followed by western blot with 66508-1-IG (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

产品信息

66508-1-IG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:6,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:175-1:700

-

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:350

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

4G3H3

抗原

SP1 Fusion Protein Ag16720 (437-785 aa encoded by BC062539)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: NLQLQAVPN SGPIIIRTPT VGPNGQVSWQ TLQLQNLQVQ NPQAQTITLA PMQGVSLGQT SSSNTTLTPI ASAASIPAGT VTVNAAQLSS MPGLQTINLS ALGTSGIQVH PIQGLPLAIA NAPGDHGAQL GLHGAGGDGI HDDTAGGEEG ENSPDAQPQA GRRTRREACT CPYCKDSEGR GSGDPGKKKQ HICHIQGCGK VYGKTSHLRA HLRWHTGERP FMCTWSYCGK RFTRSDELQR HKRTHTGEKK FACPECPKRF MRSDHLSKHI KTHQNKKGGP GVALSVGTLP LDSGAGSEGS GTATPSALIT TNMVAMEAIC PEGIARLANS GINVMQVADL QSINISGNGF (437-785 aa encoded by BC062539)

靶标信息

SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: HGNC:11205; Specificity protein 1; trans-acting transcription factor 1; Transcription factor Sp1

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基因别名: 1110003E12Rik; AA450830; AI845540; SP1; Sp1-1; TSFP1

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UniProt ID: (Human) P08047, (Mouse) O89090, (Rat) Q01714

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Entrez Gene ID: (Human) 6667, (Mouse) 20683, (Rat) 24790

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RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter sequence-specific DNA binding RNA polymerase II repressing transcription factor binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding transcription factor binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding bHLH transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding HMG box domain binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding nucleic acid binding enhancer binding RNA polymerase II transcription factor activity, sequence-specific DNA binding C2H2 zinc finger transcription factor

参与通路

regulation of transcription, DNA-templated viral process snRNA transcription from RNA polymerase II promoter positive regulation by host of viral transcription positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process regulation of phenotypic switching by transcription from RNA polymerase II promoter positive regulation of hydrogen sulfide biosynthetic process ossification in utero embryonic development trophectodermal cell differentiation liver development embryonic placenta development transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter megakaryocyte differentiation lung development enucleate erythrocyte differentiation embryonic camera-type eye morphogenesis embryonic skeletal system development embryonic process involved in female pregnancy definitive hemopoiesis