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Invitrogen
SQSTM1 Monoclonal Antibody (GT1478)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 15
SQSTM1 Antibody (MA5-27800) in ICC/IF
Immunofluorescence analysis of SQSTM1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SQSTM1 Monoclonal Antibody (GT1478) (Product # MA5-27800) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produc... View More
产品信息
MA5-27800
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
GT1478
抗原
Recombinant protein encompassing a sequence within the center region of human SQSTM1. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7, with 20% glycerol
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2735371
产品详细信息
Positive Control: HepG2, A549, H1299, HCT116, Huh-7 (untreated), Huh-7 (3 µM Thapsigargin treatment for 12 hr), Huh-7 (3 µM Thapsigargin treatment for 24 hr), HepG2 (3 µM Thapsigargin treatment for 12 hr), HepG2 (3 µM Thapsigargin treatment for 24 hr), 2C4, MEF, MNT-1
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced; oxidative stress induced like; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Sequestosome-1; STONE14; Ubiquitin-binding protein p62
基因别名: A170; FTDALS3; ORCA; OSF-6; Osi; OSIL; p60; p62; p62B; PDB3; SQSTM1; STAP; STONE14; ZIP3
UniProt ID: (Human) Q13501, (Mouse) Q64337
Entrez Gene ID: (Human) 8878, (Mouse) 18412
protein serine/threonine kinase activity
protein kinase C binding
protein binding
zinc ion binding
protein kinase binding
receptor tyrosine kinase binding
ubiquitin protein ligase binding
SH2 domain binding
identical protein binding
protein homodimerization activity
ubiquitin binding
K63-linked polyubiquitin binding
transcription cofactor activity
metal ion binding
mitophagy
positive regulation of protein phosphorylation
immune system process
protein phosphorylation
ubiquitin-dependent protein catabolic process
autophagy
apoptotic process
response to stress
protein localization
regulation of mitochondrion organization
endosomal transport
macroautophagy
positive regulation of macroautophagy
cell differentiation
intracellular signal transduction
positive regulation of apoptotic process
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of growth of symbiont in host
positive regulation of transcription from RNA polymerase II promoter
regulation of Ras protein signal transduction
protein heterooligomerization
regulation of protein complex stability
mitophagy in response to mitochondrial depolarization
regulation of nucleic acid-templated transcription
