Invitrogen

STAT4 Recombinant Rabbit Monoclonal Antibody (2H9L5)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

查看其他24个STAT4抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (8)
高级验证 (1)

STAT4 Antibody in Immunocytochemistry (ICC/IF)

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STAT4 Antibody (700185) in ICC/IF

Immunofluorescence analysis of STAT4 was done on 70% confluent log phase HeLa cells treated with IFN-gamma (50 ng/mL for 20 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT4 Rabbit monoclonal Antibody (Product # 700185) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-1100... View More

STAT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Modulation of expression of target protein by cell treatment to demonstrate antibody specificity. Immunofluorescence analysis of STAT4 using Anti-STAT4 Antibody (2H9L5), Recombinant Rabbit Monoclonal (Product # 700185) shows increased expression of STAT4 in HeLa cells treated with INF gamma. {TM}

产品信息

700185

应用

建议稀释比

已发表文章

免疫印迹 (WB)

3-5 µg/mL

免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

流式细胞分析 (Flow)

1:100-1:500

酶联免疫吸附实验 (ELISA)

1-5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

2H9L5

抗原

A peptide corresponding to amino acids 730-748 of Q14765.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2532296

产品详细信息

This antibody is predicted to react with chicken, equine and porcine based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin 12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study in mouse has shown that in response to viral infection IFN-a/b activation of STAT4 is required for IFN-g production.

仅用于科研。不用于诊断过程。未经明确授权不得转售。