Invitrogen

STAT5 alpha/beta Monoclonal Antibody (ST5-8F7)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3个已发表图片

6 篇参考文献

查看其他12个STAT5 alpha/beta抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

STAT5 alpha/beta Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

STAT5 alpha/beta Antibody (33-5900) in ICC/IF

Immunofluorescence analysis of STAT5 was performed using 0.7 % confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with STAT5 Mouse Monoclonal antibody (Product # 33-5900) at 5 µg/mL in 0.1% BSA and incubated for 3 hours at room temper... View More

STAT5 alpha/beta Antibody in Western Blot (WB)

Product Image Gallery

产品信息

33-5900

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ST5-8F7

抗原

A peptide corresponding to a conserved amino acid sequence found near the carboxy-terminus of murine STAT5. This peptide encompasses the conserved C-terminal tyrosine phosphorylation site (Y-694) of murine STAT5

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533129

靶标信息

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of, many cell ligands, such as IL-2, IL-3, IL-7, GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/ BCL-X(L), which suggests the antiapoptotic function of this gene in cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia.

Leukemia

Degryse S,de Bock CE,Demeyer S,Govaerts I,Bornschein S,Verbeke D,Jacobs K,Binos S,Skerrett-Byrne DA,Murray HC,Verrills NM,Van Vlierberghe P,Cools J,Dun MD

Published figure using STAT5 alpha/beta monoclonal antibody (Product # 33-5900) in Western Blot

Thu Mar 01 00:00:00 EST 2018

Common γ-chain cytokine signaling is required for macroautophagy induction during CD4+ T-cell activation.

Autophagy

Botbol Y,Patel B,Macian F

Published figure using STAT5 alpha/beta monoclonal antibody (Product # 33-5900) in Western Blot

Tue Sep 13 00:00:00 EDT 2016

Common γ-chain cytokine signaling is required for macroautophagy induction during CD4+ T-cell activation.

Autophagy

Botbol Y,Patel B,Macian F

Published figure using STAT5 alpha/beta monoclonal antibody (Product # 33-5900) in Western Blot

Tue Sep 13 00:00:00 EDT 2016

Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).

Oncogene

Duensing A,Medeiros F,McConarty B,Joseph NE,Panigrahy D,Singer S,Fletcher CD,Demetri GD,Fletcher JA

Thu May 13 00:00:00 EDT 2004

Viral FLIP impairs survival of activated T cells and generation of CD8+ T cell memory.

Journal of immunology (Baltimore, Md. : 1950)

Wu Z,Roberts M,Porter M,Walker F,Wherry EJ,Kelly J,Gadina M,Silva EM,DosReis GA,Lopes MF,O'Shea J,Leonard WJ,Ahmed R,Siegel RM

Sat May 15 00:00:00 EDT 2004

PU.1 supports proliferation of immature erythroid progenitors.

Leukemia research

Fisher RC,Slayton WB,Chien C,Guthrie SM,Bray C,Scott EW

33-5900 was used in western blot to propose that PU.1 has an important role in regulating the proliferation of immature erythroid progenitors

Thu Jan 01 00:00:00 EST 2004

The Epstein-Barr virus latent membrane protein 1 putative Janus kinase 3 (JAK3) binding domain does not mediate JAK3 association or activation in B-lymphoma or lymphoblastoid cell lines.

Journal of virology

Higuchi M,Kieff E,Izumi KM

Tue Jan 01 00:00:00 EST 2002

生物信息学

蛋白别名: Mammary gland factor; mammary gland factor STAT5A; mammary gland factor STAT5B; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5A; Signal transducer and activator of transcription 5B; STA5B; STAT; transcription factor STAT5B

查看更多收起

基因别名: AA959963; MGF; Mpf; STAT5; STAT5A; STAT5B

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功能

DNA binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding protein phosphatase binding glucocorticoid receptor binding protein dimerization activity double-stranded DNA binding sequence-specific DNA binding DNA-binding transcription factor

参与通路

STAT5 alpha/beta Monoclonal Antibody (ST5-8F7)

STAT5 alpha/beta Antibody (33-5900) in ICC/IF

产品信息

33-5900

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

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