Invitrogen

STEAP1 Polyclonal Antibody

STEAP1 Antibody in Immunocytochemistry (ICC/IF)

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STEAP1 Antibody (PA5-103171) in ICC/IF

Immunocytochemistry analysis of STEAP1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with STEAP1 polyclonal antibody (Product # PA5-103171) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

STEAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-103171

应用

建议稀释比

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免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human STEAP1(Accession Q9UHE8), corresponding to amino acid residues I7-C57.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2852541

产品详细信息

Antibody detects endogenous levels of total STEAP1.

靶标信息

STEAP1 is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. The gene product is predicted to be a six-transmembrane protein, and was shown to be a cell surface antigen significantly expressed at cell-cell junctions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。