Invitrogen

STX17 Polyclonal Antibody

STX17 Antibody in Immunocytochemistry (ICC/IF)

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STX17 Antibody (PA5-40127) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of STX17 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: STX17 Polyclonal Antibody (Product # PA5-40127) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

STX17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-40127

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human STX17.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.47 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2608436

产品详细信息

Recommended positive controls: 293T, A431, HeLa, and HepG2 cells.

Predicted reactivity: Mouse (89%), Rat (86%), Rhesus Monkey (99%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. STX17 is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi. (Uniprot)

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Glycan degradation promotes macroautophagy.

Proceedings of the National Academy of Sciences of the United States of America

Baudot AD,Wang VM,Leach JD,O'Prey J,Long JS,Paulus-Hock V,Lilla S,Thomson DM,Greenhorn J,Ghaffar F,Nixon C,Helfrich MH,Strathdee D,Pratt J,Marchesi F,Zanivan S,Ryan KM

Published figure using STX17 polyclonal antibody (Product # PA5-40127) in Western Blot

Tue Jun 28 00:00:00 EDT 2022

The CD36 Ligand-Promoted Autophagy Protects Retinal Pigment Epithelial Cells from Oxidative Stress.

Oxidative medicine and cellular longevity

Dorion MF,Mulumba M,Kasai S,Itoh K,Lubell WD,Ong H

PA5-40127 was used in Western Blot, Immunocytochemistry to report for the first time an autophagy-dependent protection of RPE cells from oxidative stress by a CD36 ligand.

Wed Jun 09 00:00:00 EDT 2021

The CD36 Ligand-Promoted Autophagy Protects Retinal Pigment Epithelial Cells from Oxidative Stress.

Oxidative medicine and cellular longevity

Dorion MF,Mulumba M,Kasai S,Itoh K,Lubell WD,Ong H

PA5-40127 was used in Western Blot, Immunocytochemistry to report for the first time an autophagy-dependent protection of RPE cells from oxidative stress by a CD36 ligand.

Wed Jun 09 00:00:00 EDT 2021

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.

Nature communications

Xian H,Yang Q,Xiao L,Shen HM,Liou YC

PA5-40127 was used in Immunocytochemistry-immunoflourescence to demonstrate that the SNARE protein Syntaxin 17 initiates mitophagy upon depletion of outer mitochondrial membrane protein Fis1.

Fri May 03 00:00:00 EDT 2019

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.

Nature communications

Xian H,Yang Q,Xiao L,Shen HM,Liou YC

PA5-40127 was used in Immunocytochemistry-immunoflourescence to demonstrate that the SNARE protein Syntaxin 17 initiates mitophagy upon depletion of outer mitochondrial membrane protein Fis1.