Invitrogen

SUMO1 Monoclonal Antibody (T.243.0)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (6)
高级验证 (1)

SUMO1 Antibody in Immunocytochemistry (ICC/IF)

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SUMO1 Antibody (MA5-14877) in ICC/IF

Immunofluorescence analysis of SUMO1 (Small ubiquitin-related modifier 1) was performed using 80% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SUMO1 Monoclonal Antibody (T.243.0) (Product # MA5-14877) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, A... View More

SUMO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SUMO1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with SUMO1, SUMO2 and SUMO3 siRNA, separately, and decrease in signal intensity was observed only in SUMO1 siRNA transfected cells and not in the other two SUMOs in ICC application using Anti-SUMO1 Monoclonal Antibody (T.243.0) (Product # MA5-14877). {KD}

产品信息

MA5-14877

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:50

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

1:50

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

T.243.0

抗原

Synthetic peptide from the amino terminus of human SUMO-1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

23 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10979860

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved, for instance, in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. SUMO1 may also regulate a network of genes involved in palate development. Mutations in the gene can result in non-syndromic orofacial cleft 10.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CG4494-PA; GAP modifying protein 1; GAP-modifying protein 1; GMP1; LD07775p; OTTHUMP00000202403; OTTHUMP00000202404; OTTHUMP00000202405; OTTHUMP00000202406; OTTHUMP00000202409; OTTHUMP00000202410; Sentrin; Sentrin-1; Small ubiquitin-related modifier 1; small ubiquitin-related modifier-1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1; Smt3C; SUMO-1; SUMO-1 related peptidase; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1; ubiquitin-like 1 (sentrin); Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1

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基因别名: DAP1; GMP1; OFC10; OK/SW-cl.43; PIC1; SENP2; SENTRIN; SMT3; SMT3C; SMT3H3; SMTP3; SUMO-1; SUMO1; UBL1

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UniProt ID: (Human) P63165, (Mouse) P63166, (Rat) Q5I0H3

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Entrez Gene ID: (Human) 7341, (Mouse) 22218, (Rat) 301442

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功能

protein binding transcription factor binding potassium channel regulator activity protein tag ubiquitin protein ligase binding ion channel binding poly(A) RNA binding SUMO transferase activity

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