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SaCas9 Antibody (A01951-40) in ICC/IF
Immunofluorescence staining of GenCRISPR™ SaCas9 Antibody (11C12) (GenScript, A01951-40) with SaCas9 in Hela cells. HeLa cells transfected with PX458 (SaCas9(BB)-2A-GFP) (colored green) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min. The cells were then incubated with GenCRISPR™ SaCas9 Antibody (11C12) (GenScript, A01951-40) at 0.5 µg/mL mouse IgG at 0.5 µg/mL at room temperature for 2h, followed by a further incubation at 37 °C for 1h with Goat Anti-Mouse IgG Antibody (H&L) [ifluor 555], pAb (GenScript) (colo... View More
产品信息
A01951-40
应用
建议稀释比
已发表文章
产品规格
种属反应
Bacteria
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
11C12
抗原
Recombinant Staphylococcus aureus CRISPR/Cas9
偶联物
形式
Lyophilized
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.02% sodium azide
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein 9 system provides a robust and multiplexable genome editing tool, enabling researchers to precisely manipulate specific genomic elements, and facilitating the elucidation of target gene function in biology and diseases. CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The best characterized CRISPR-associated nucleases are the Cas9 proteins from Streptococcus pyogenes and Staphylococcus aureus. It is important that the precision of transfection and the level of Cas9 expression should be controlled strictly during the editing processes by using specific anti-CRISPR/Cas9 antibodies.
靶标信息
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cas9; CASP 9; Caspase-9 precursor; CASPASE-9c; Caspase9; CRISPR-Cas9; CRISPR9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus
