Invitrogen

Sall1 Monoclonal Antibody (NRNSTNX), eBioscience™

Sall1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Sall1 Antibody (14-9729-82) in IHC (F)

Immunohistochemistry analysis of Sall1 in mouse brain tissue. Coronal cryosections of mouse brain tissue were fixed with ice cold acetone for 10 minutes and blocked with 10% mouse serum for 1hr at room temperature. The sections were stained with 1.25 µg /ml Sall1 Monoclonal Antibody overnight at 4C and then labeled with Goat anti-Rat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

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产品信息

14-9729-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 μg/mL

免疫组化 (IHC)

- -

免疫组化（冰冻） (IHC (F))

1.25 μg/mL

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

NRNSTNX

抗原

C-terminal fragment of the protein (aa-805-1322)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2848275

产品详细信息

Description: This NRNSTNX monoclonal antibody recognizes mouse Sall1. This clone has been reported to be successfully used in flow cytometry, native gel electrophoresis followed by western blot and immunohistochemistry of frozen tissue.

Applications Reported: This NRNSTNX antibody has been reported for use in native gel electrophoresis followed by western blot and in immunohistochemical staining.

Applications Tested: This NRNSTNX antibody has been tested by western blot (native) of mouse brain. This may be used at less than or equal to 5 µg/mL. This clone is not recommended for detection of denatured Sall1.

Applications Tested: This NRNSTNX antibody has been tested by immunohistochemical staining of acetone fixed frozen sections from mouse brain. This may be used at less than or equal to 1.25 µg/mL.

(Fluorochrome-conjugated NRNSTNX is recommended for use in flow cytometry)

靶标信息

Sall1, which encodes a zinc finger protein, functions as a transcriptional repressor and interacts physically with histone deacetylase and other components of the chromatin remodeling NuRD complex. It is unknown whether the transcriptional repression is solely dependent on histone deacetylase activity. Gene expression profiling has identified Sall1 as a microglial signature gene. Microglia are the resident macrophages of the central nervous system (CNS). Sall1 is also expressed in abundance in the mesenchyme-derived structure from condensed mesenchyme, S-comma-shaped bodies, to renal tubules and podocytes. Sall1 has been identified as a key transcription factor in self-renewal renal progenitor cells. Sall1 is required to maintain the stemness of nephron progenitor cells by restraining their differentiation into renal vesicles. Defects in SALL1 are the cause of Townes-Brocks syndrome as well as bronchio-oto-renal syndrome. Heterozygous mutations of human SALL1 leading to Townes-Brocks syndrome features dysplastic ears, preaxial polydactyly, imperforate anus, and less commonly, kidney and heart anomalies (Kohlhase et al. 1998). Two transcript variants encoding different isoforms have been found for this gene.

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