Invitrogen

Sall1 Monoclonal Antibody (NRNSTNX), Alexa Fluor™ 488, eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

查看其他7个SALL1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
高级验证 (1)

图: 1 / 2

Sall1 Antibody (53-9729-82) in Flow

Demyelinated BALB/c mouse brain cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD11b Monoclonal Antibody, PE (Product # 12-0112-82) and 0.5 µg of Rat IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4031-80) (left) or 0.5 µg of Sall1 Monoclonal Antibody, Alexa Fluor 488 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

Product Image Gallery

Sall1 Antibody — Staining of mouse brain cells. As expected, clone NRNSTNX stains mouse microglia (CD11b+ CD45 intermediate; purple) but not CD45 high CD11b+ cells (blue) in the brain. Details: Demyelinated mouse brain single cell suspension was stained with Fixable Viability Dye eF450 and either Rat IgG2a isotype control (left histogram), or clone NRNSTNX (right histogram). Viable cells were used for analysis. {RE}

产品信息

53-9729-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1.0 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

NRNSTNX

抗原

C-terminal fragment of the protein (aa-805-1322)

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偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2848447

产品详细信息

Description: This NRNSTNX monoclonal antibody recognizes mouse Sall1.

Applications Reported: This NRNSTNX antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This NRNSTNX antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse brain cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

靶标信息

Sall1, which encodes a zinc finger protein, functions as a transcriptional repressor and interacts physically with histone deacetylase and other components of the chromatin remodeling NuRD complex. It is unknown whether the transcriptional repression is solely dependent on histone deacetylase activity. Gene expression profiling has identified Sall1 as a microglial signature gene. Microglia are the resident macrophages of the central nervous system (CNS). Sall1 is also expressed in abundance in the mesenchyme-derived structure from condensed mesenchyme, S-comma-shaped bodies, to renal tubules and podocytes. Sall1 has been identified as a key transcription factor in self-renewal renal progenitor cells. Sall1 is required to maintain the stemness of nephron progenitor cells by restraining their differentiation into renal vesicles. Defects in SALL1 are the cause of Townes-Brocks syndrome as well as bronchio-oto-renal syndrome. Heterozygous mutations of human SALL1 leading to Townes-Brocks syndrome features dysplastic ears, preaxial polydactyly, imperforate anus, and less commonly, kidney and heart anomalies (Kohlhase et al. 1998). Two transcript variants encoding different isoforms have been found for this gene.

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