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Slo2.2 Antibody (MA5-45434) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Slo2.2 monoclonal antibody (Product # MA5-45434) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Slo2.2 antibody (D) Composite.
产品信息
MA5-45434
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat,
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N3/26
抗原
Fusion protein amino acids 1168-1237 of rat Slo2.2 (Slack)
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931888
产品详细信息
1 µg/mL of MA5-45434 was sufficient for detection of Slo2.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa. Weak human detection.approximately Does not cross-react with KCNT2/Slo2.1/Slick.
靶标信息
Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a sodium-activated potassium channel subunit which is thought to function in ion conductance and developmental signaling pathways. Mutations in this gene cause the early-onset epileptic disorders, malignant migrating partial seizures of infancy and autosomal dominant nocturnal frontal lobe epilepsy. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: KCa4.1; KCa4.1KIAA1422FLJ41282; Potassium channel subfamily T member 1; potassium channel subunit (Slack); potassium channel, sodium activated subfamily T, member 1; potassium channel, subfamily T, member 1; Sequence like a calcium-activated K+ channel; Sequence like a calcium-activated potassium channel subunit; Slo2.2
基因别名: bA100C15.2; C030030G16Rik; EIEE14; ENFL5; KCa4.1; KCNT1; KIAA1422; rSlo2; SLACK; slo2; Slo2.2
UniProt ID: (Human) Q5JUK3, (Mouse) Q6ZPR4, (Rat) Q9Z258
Entrez Gene ID: (Human) 57582, (Mouse) 227632, (Rat) 60444
