Invitrogen

Sortilin Polyclonal Antibody

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Sortilin Antibody (PA5-96865) in WB

Western blot analysis of Sortilin in extracts of various cell lines. Samples were incubated with Sortilin Polyclonal antibody (Product # PA5-96865) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

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产品信息

PA5-96865

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:1,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 785-823 of human SORT1 (NP_0029503)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.59 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2808667

产品详细信息

Positive Samples: A-549, Jurkat; Cellular Location: Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Extracellular side, Golgi apparatus, Golgi stack membrane, Lysosome membrane, Membrane, Nucleus membrane, Single-pass type I membrane protein

Immunogen sequence: GRFLVHRYSV LQQHAEANGV DGVDALDTAS HTNKSGYHD

靶标信息

Sortilin functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Sortilin is required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). It is also required for protein transport from the Golgi apparatus to the endosomes. Sortilin promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also, Sortilin acts as a receptor for neurotensin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。