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Strep Tag II Antibody (MA5-42540) in WB
Western blot analysis of Strep-Tag II on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Strep Tag II Monoclonal Antibody (Product # MA5-42540) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Strep-Tag II protein transfected 293F cells lysate. Lane 2: Non-transfected 293F cells lysate.
产品信息
MA5-42540
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
A5D11
抗原
Synthetic peptide corresponding to Strep-tag II
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Protein G
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2911681
产品详细信息
Positive Control: Strep-Tag II protein transfected 293F cells lysate, Non-transfected 293F cells lysate.
靶标信息
The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Streptavidin; Streptomyces
