Proteintech

Synaptotagmin-1 Polyclonal Antibody

Synaptotagmin-1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

Synaptotagmin-1 Antibody (14511-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 14511-1-AP (SynAPtotagmin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Synaptotagmin-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Synaptotagmin-1 Antibody in Western Blot (WB)

Synaptotagmin-1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

产品信息

14511-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看8篇已发表文章 8篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫细胞化学 (ICC/IF)

1:50-1:500

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

0.5-4.0 µg

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

不适用 (N/A)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synaptotagmin-1 Fusion Protein Ag5970 (77-422 aa encoded by BC058917)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.

Immunogen sequence: FCICKKCLF KKKNKKKGKE KGGKNAINMK DVKDLGKTMK DQALKDDDAE TGLTDGEEKE EPKEEEKLGK LQYSLDYDFQ NNQLLVGIIQ AAELPALDMG GTSDPYVKVF LLPDKKKKFE TKVHRKTLNP VFNEQFTFKV PYSELGGKTL VMAVYDFDRF SKHDIIGEFK VPMNTVDFGH VTEEWRDLQS AEKEEQEKLG DICFSLRYVP TAGKLTVVIL EAKNLKKMDV GGLSDPYVKI HLMQNGKRLK KKKTTIKKNT LNPYYNESFS FEVPFEQIQK VQVVVTVLDY DKIGKNDAIG KVFVGYNSTG AELRHWSDML ANPRRPIAQW HTLQVEEEVD AMLAVKK (77-422 aa encoded by BC058917)

靶标信息

Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus.

Cell reports

Wen L,Yang X,Wu Z,Fu S,Zhan Y,Chen Z,Bi D,Shen Y

Tue Apr 25 00:00:00 EDT 2023

Long-read sequencing and profiling of RNA-binding proteins reveals the pathogenic mechanism of aberrant splicing of an SCN1A poison exon in epilepsy.

bioRxiv : the preprint server for biology

Happ HC,Schneider PN,Hong JH,Goes E,Bandouil M,Biar CG,Ramamurthy A,Reese F,Engel K,Weckhuysen S,Scheffer IE,Mefford HC,Calhoun JD,Carvill GL

Thu May 04 00:00:00 EDT 2023

A Class I HDAC Inhibitor BG45 Alleviates Cognitive Impairment through the CaMKII/ITPKA/Ca²⁺ Signaling Pathway.

Pharmaceuticals (Basel, Switzerland)

Liu J,Zhang C,Wang J,Huang Y,Shen D,Hu Y,Chu H,Yu X,Zhang L,Ma H

Mon Nov 28 00:00:00 EST 2022

A Class I HDAC Inhibitor BG45 Alleviates Cognitive Impairment through the CaMKII/ITPKA/Ca²⁺ Signaling Pathway.

Pharmaceuticals (Basel, Switzerland)

Liu J,Zhang C,Wang J,Huang Y,Shen D,Hu Y,Chu H,Yu X,Zhang L,Ma H

Mon Nov 28 00:00:00 EST 2022

Discrimination of the Activity of Low-Affinity Wild-Type and High-Affinity Mutant Recombinant BoNT/B by a SIMA Cell-Based Reporter Release Assay.

Toxins

Neuschäfer-Rube F,Pathe-Neuschäfer-Rube A,Püschel GP

Mon Jan 17 00:00:00 EST 2022

ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells.

Theranostics

Pan T,Liu J,Xu S,Yu Q,Wang H,Sun H,Wu J,Zhu Y,Zhou J,Zhu Y

Wed Apr 21 00:00:00 EDT 2021

Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Sancho-Knapik S,Pastor O,Barranquero C,Herrera Marcos LV,Guillén N,Arnal C,Gascón S,Navarro MA,Rodríguez-Yoldi MJ,Busto R,Lasunción MA,Osada J

Tue Dec 01 00:00:00 EST 2020

Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Sancho-Knapik S,Pastor O,Barranquero C,Herrera Marcos LV,Guillén N,Arnal C,Gascón S,Navarro MA,Rodríguez-Yoldi MJ,Busto R,Lasunción MA,Osada J

Tue Dec 01 00:00:00 EST 2020

Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain.

Biochimica et biophysica acta. Proteins and proteomics

Zeng HL,Yu FL,Zhang Z,Yang Q,Jin S,He X,Chen X,Shen Y,Cheng L,Guo L,Xu F

Thu Feb 01 00:00:00 EST 2018

Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain.

Biochimica et biophysica acta. Proteins and proteomics

Zeng HL,Yu FL,Zhang Z,Yang Q,Jin S,He X,Chen X,Shen Y,Cheng L,Guo L,Xu F

Thu Feb 01 00:00:00 EST 2018

A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13.

Frontiers in molecular neuroscience

Li Y,Wang S,Li T,Zhu L,Xu Y,Ma C

Tue Sep 29 00:00:00 EDT 2020

A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13.

Frontiers in molecular neuroscience

Li Y,Wang S,Li T,Zhu L,Xu Y,Ma C

Tue Sep 29 00:00:00 EDT 2020

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Journal of proteomics

Zeng HL,Rao X,Zhang LK,Zhao X,Zhang WP,Wang J,Xu F,Guo L

Tue Sep 23 00:00:00 EDT 2014

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Journal of proteomics

Zeng HL,Rao X,Zhang LK,Zhao X,Zhang WP,Wang J,Xu F,Guo L

Tue Sep 23 00:00:00 EDT 2014

Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.

PloS one

Tian H,Wang L,Cai R,Zheng L,Guo L

Fri Aug 28 00:00:00 EDT 2015

Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.

PloS one

Tian H,Wang L,Cai R,Zheng L,Guo L

Fri Aug 28 00:00:00 EDT 2015

Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.

PloS one

Tian H,Wang L,Cai R,Zheng L,Guo L

Fri Aug 28 00:00:00 EDT 2015

Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.

PloS one

Tian H,Wang L,Cai R,Zheng L,Guo L

Fri Aug 28 00:00:00 EDT 2015

生物信息学

蛋白别名: DKFZp781D2042; FLJ42519; p65; SV DKFZp781D2042; SV SYT; Syb1; synaptotagmin 1; synaptotagmin 2; Synaptotagmin I; Synaptotagmin-1; SYT2; SytI

查看更多收起

基因别名: AW124717; G630098F17Rik; P65; SVP65; SYT; SYT1; SytI

查看更多收起

UniProt ID: (Human) P21579, (Mouse) P46096

查看更多收起

Entrez Gene ID: (Human) 6857, (Mouse) 20979

查看更多收起

功能